The influence of ambient temperature and polyphenols from plant leaves on growth and the response to oxidative and nitrosative stress in African nightcrawler earthworm, Eudrilus eugeniae (Kinberg, 1867)

Abstract

Earthworms are one of the organisms that may be affected by climate change. Finding ways to help them deal with this problem is, therefore, important and necessary. The objective of this experiment was to understand the influence of ambient temperature and polyphenols from mulberry (Morus alba L.), almond (Terminalia catappa L.) and cassava (Manihot esculenta (L.) Crantz) leaves on growth, ferric reducing antioxidant power (FRAP), malondialdehyde (MDA), hydrogen peroxide (H₂O₂) and nitric oxide (NO) concentration of the African night crawler, Eudrilus eugeniae (Kinberg, 1867) earthworm. The earthworms were cultured in 2 different conditions of ambient temperature, and in 4 types of substrate i.e. dairy cow faeces (BS), dairy cow faeces + mulberry leaves (BS + MA), almond leaves (BS + TC), and cassava leaves (BS + ME), respectively. At week 2 of the experiment, body weight, FRAP, MDA, H₂O₂ and NO were measured in the earthworms. It was found that the body weight gain (BWG) of the earthworms cultured in BS at cyclical temperature (26 + 1^oC - 34 + 1^oC - 26 + 1^oC, CyT) was higher than the constant temperature (26 ± 1 °C, CoT) (P < 0.05). FRAP of earthworms cultured in BS + TC was higher than in other groups (P < 0.05). MDA of earthworms cultured at CyT was higher than ambient temperature at CoT (P < 0.05). At CyT, the MDA of earthworms cultured in BS + MA was higher than that of those cultured in BS, BS + TC and BS + ME (P < 0.05). NO of earthworms at CoT was higher than at CyT(P < 0.05). At CoT, the NO of earthworms cultured in BS + TC was lower than that of those cultured in BS + MA and BS + ME (P < 0.05). H₂O₂ of earthworms at CoT was higher than those at CyT (P < 0.05). The level of H₂O₂ of the earthworms cultured in BS + ME at CoT was higher than at CyT (P < 0.05). In addition, the H₂O₂ of earthworms cultured in both ambient temperatures and cultured in BS + MA was higher than the other groups (P < 0.05). These phenomena indicated that low and high ambient temperatures induced nitrosative and oxidative stress in earthworms, respectively. Mulberry leaves are toxic to earthworms. On the other hand, almond leaves could reduce nitrosative stress in earthworms. While at the CoT, cassava leaves induced the production of H₂O₂ in the earthworms.

Keywords: 1867); African nightcrawler earthworm; Ambient temperature; Eudrilus eugeniae (Kinberg; Nitrosative stress; Oxidative stress; Polyphenols.