Production and characterization of bioemulsifier by Parapedobacter indicus

Anushka Devale; Rupali Sawant; Karishma Pardesi; Kahkashan Perveen; Mehrun NIsha Khanam; Yogesh Shouche; Shilpa Mujumdar

doi:10.3389/fmicb.2023.1111135

Production and characterization of bioemulsifier by Parapedobacter indicus

Front Microbiol. 2023 Feb 16:14:1111135. doi: 10.3389/fmicb.2023.1111135. eCollection 2023.

Authors

Anushka Devale¹, Rupali Sawant¹, Karishma Pardesi², Kahkashan Perveen³, Mehrun NIsha Khanam⁴, Yogesh Shouche⁵, Shilpa Mujumdar¹

Affiliations

¹ Department of Microbiology, P.E.S. Modern College of Arts, Science and Commerce (Autonomous), Pune, India.
² Department of Microbiology, Savitribai Phule Pune University, Pune, India.
³ Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia.
⁴ School of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul, Republic of Korea.
⁵ School of Arts and Sciences, Azim Premji University, Bengaluru, India.

Abstract

The current study evaluated Parapedobacter indicus MCC 2546 for its potential to produce a bioemulsifier (BE). Screening methods performed for BE production by P. indicus MCC 2546 showed good lipase activity, positive drop collapse test, and oil-spreading activity. Furthermore, it showed maximum emulsification activity (225 EU/ml) and emulsification index (E₂₄ 50%) at 37°C in Luria Bertani broth at 72 h with olive oil as a substrate. The optimal pH and NaCl concentration for maximum emulsification activity were 7 and 1%, respectively. P. indicus MCC 2546 lowered the surface tension of the culture medium from 59.65 to 50.42 ± 0.78 mN/m. BE produced was composed of 70% protein and 30% carbohydrate, which showed the protein-polysaccharide nature of the BE. Furthermore, Fourier transform infrared spectroscopy analysis confirmed the same. P. indicus MCC 2546 showed a catecholate type of siderophore production. This is the first report on BE and siderophore production by the genus Parapedobacter.

Keywords: BS/BE; Parapedobacter; bioemulsifier; bioremediation; heavy metal resistance.