Antiproliferative activity of antimicrobial peptides and bioactive compounds from the mangrove Glutamicibacter mysorens

Yalpi Karthik; Manjula Ishwara Kalyani; Srinivasa Krishnappa; Ramakrishna Devappa; Chengeshpur Anjali Goud; Krishnaveni Ramakrishna; Muneeb Ahmad Wani; Mohamed Alkafafy; Maram Hussen Abduljabbar; Amal S Alswat; Samy M Sayed; Muntazir Mushtaq

doi:10.3389/fmicb.2023.1096826

Antiproliferative activity of antimicrobial peptides and bioactive compounds from the mangrove Glutamicibacter mysorens

Front Microbiol. 2023 Feb 17:14:1096826. doi: 10.3389/fmicb.2023.1096826. eCollection 2023.

Affiliations

¹ Department of Studies and Research in Microbiology, Mangalore University, Mangalore, Karnataka, India.
² Department of Studies and Research in Biochemistry, Mangalore University, Mangalore, Karnataka, India.
³ Dr. C.D Sagar Centre for Life Sciences, Biotechnology Department, Dayananda Sagar College of Engineering, Dayananda Sagar Institutions, Bengaluru, India.
⁴ Department of Plant Biotechnology, School of Agricultural Sciences, Malla Reddy University, Hyderabad, India.
⁵ Department of Studies and Research in Microbiology, Vijayanagara Sri Krishnadevaraya University, Ballari, Karnataka, India.
⁶ Division of Floriculture, Sher-e-Kashmir University of Agricultural Sciences and Technology, Srinagar, Jammu and Kashmir, India.
⁷ Department of Cytology and Histology, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Egypt.
⁸ Department of Pharmacology and Toxicology, College of Pharmacy, Taif University, Taif, Saudi Arabia.
⁹ Department of Biotechnology, College of Science, Taif University, Taif, Saudi Arabia.
¹⁰ Department of Economic Entomology and Pesticides, Faculty of Agriculture, Cairo University, Giza, Egypt.
¹¹ ICAR-National Bureau of Plant Genetic Resources, Division of Germplasm Evaluation, New Delhi, India.
¹² MS Swaminathan School of Agriculture, Shoolini University of Biotechnology and Management, Bajhol, Himachal Pradesh, India.

Abstract

The Glutamicibacter group of microbes is known for antibiotic and enzyme production. Antibiotics and enzymes produced by them are important in the control, protection, and treatment of chronic human diseases. In this study, the Glutamicibacter mysorens (G. mysorens) strain MW647910.1 was isolated from mangrove soil in the Mangalore region of India. After optimization of growth conditions for G. mysorens on starch casein agar media, the micromorphology of G. mysorens was found to be spirally coiled spore chain, each spore visualized as an elongated cylindrical hairy appearance with curved edges visualized through Field Emission Scanning Electron Microscopy (FESEM) analysis. The culture phenotype with filamentous mycelia, brown pigmentation, and ash-colored spore production was observed. The intracellular extract of G. mysorens characterized through GCMS analysis detected bioactive compounds reported for pharmacological applications. The majority of bioactive compounds identified in intracellular extract when compared to the NIST library revealed molecular weight ranging below 1kgmole^-1. The Sephadex G-10 could result in 10.66 fold purification and eluted peak protein fraction showed significant anticancer activity on the prostate cancer cell line. Liquid Chromatography-Mass Spectrometry (LC-MS) analysis revealed Kinetin-9-ribose and Embinin with a molecular weight below 1 kDa. This study showed small molecular weight bioactive compounds produced from microbial origin possess dual roles, acting as antimicrobial peptides (AMPs) and anticancer peptides (ACPs). Hence, the bioactive compounds produced from microbial origin are a promising source of future therapeutics.

Keywords: FESEM; Glutamicibacter mysorens; anticancer; chromatography; mangrove soil; microbial peptides.