New perspective: Symbiotic pattern and assembly mechanism of Cantharellus cibarius-associated bacteria

Wei Ge; Yulian Ren; Chunbo Dong; Qiuyu Shao; Yanmin Bai; Zhaoying He; Ting Yao; Yanwei Zhang; Guosheng Zhu; Sunil Kumar Deshmukh; Yanfeng Han

doi:10.3389/fmicb.2023.1074468

New perspective: Symbiotic pattern and assembly mechanism of Cantharellus cibarius-associated bacteria

Front Microbiol. 2023 Feb 16:14:1074468. doi: 10.3389/fmicb.2023.1074468. eCollection 2023.

Authors

Wei Ge¹, Yulian Ren¹, Chunbo Dong¹, Qiuyu Shao¹, Yanmin Bai¹, Zhaoying He¹, Ting Yao², Yanwei Zhang³, Guosheng Zhu⁴, Sunil Kumar Deshmukh⁵, Yanfeng Han¹

Affiliations

¹ Institute of Fungus Resources, Department of Ecology/Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-Bioengineering, Guizhou University, Guiyang, Guizhou, China.
² Analysis and Test Center, Huangshan University, Huangshan, China.
³ School of Biological Sciences, Guizhou Education University, Guiyang, Guizhou, China.
⁴ Guizhou Key Laboratory of Edible Fungi Breeding, Institute of Crop Germplasm Resources, Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou, China.
⁵ TERI-Deakin Nano Biotechnology Centre, The Energy and Resources Institute, New Delhi, India.

Abstract

Cantharellus cibarius, an ectomycorrhizal fungus belonging to the Basidiomycetes, has significant medicinal and edible value, economic importance, and ecological benefits. However, C. cibarius remains incapable of artificial cultivation, which is thought to be due to the presence of bacteria. Therefore, much research has focused on the relationship between C. cibarius and bacteria, but rare bacteria are frequently overlooked, and symbiotic pattern and assembly mechanism of the bacterial community associated with C. cibarius remain unknown. In this study, the assembly mechanism and driving factors of both abundant and rare bacterial communities of C. cibarius were revealed by the null model. The symbiotic pattern of the bacterial community was examined using a co-occurrence network. Metabolic functions and phenotypes of the abundant and rare bacteria were compared using METAGENassist2, and the impacts of abiotic variables on the diversity of abundant and rare bacteria were examined using partial least squares path modeling. In the fruiting body and mycosphere of C. cibarius, there was a higher proportion of specialist bacteria compared with generalist bacteria. Dispersal limitation dominated the assembly of abundant and rare bacterial communities in the fruiting body and mycosphere. However, pH, 1-octen-3-ol, and total phosphorus of the fruiting body were the main driving factors of bacterial community assembly in the fruiting body, while available nitrogen and total phosphorus of the soil affected the assembly process of the bacterial community in the mycosphere. Furthermore, bacterial co-occurrence patterns in the mycosphere may be more complex compared with those in the fruiting body. Unlike the specific potential functions of abundant bacteria, rare bacteria may provide supplementary or unique metabolic pathways (such as sulfite oxidizer and sulfur reducer) to enhance the ecological function of C. cibarius. Notably, while volatile organic compounds can reduce mycosphere bacterial diversity, they can increase fruiting body bacterial diversity. Findings from this study further, our understanding of C. cibarius-associated microbial ecology.

Keywords: Cantharellus cibarius; community assembly; diversity; microbial interaction; mycosphere; volatile organic compounds.