Genome-wide analysis of 14-3-3 gene family in four gramineae and its response to mycorrhizal symbiosis in maize

Yanping Wang; Qiang Xu; Hanchen Shan; Ying Ni; Minyan Xu; Yunjian Xu; Beijiu Cheng; Xiaoyu Li

doi:10.3389/fpls.2023.1117879

Genome-wide analysis of 14-3-3 gene family in four gramineae and its response to mycorrhizal symbiosis in maize

Front Plant Sci. 2023 Feb 17:14:1117879. doi: 10.3389/fpls.2023.1117879. eCollection 2023.

Authors

Yanping Wang¹, Qiang Xu¹, Hanchen Shan¹, Ying Ni¹, Minyan Xu¹, Yunjian Xu², Beijiu Cheng¹, Xiaoyu Li¹

Affiliations

¹ National Engineering Laboratory of Crop Stress Resistance Breeding, Anhui Agricultural University, Hefei, China.
² Yunnan Key Laboratory of Plant Reproductive Adaptation and Evolutionary Ecology and Institute of Biodiversity, School of Ecology and Environmental Science, Yunnan University, Kunming, China.

Abstract

14-3-3 proteins (regulatory protein family) are phosphate serine-binding proteins. A number of transcription factors and signaling proteins have been shown to bind to the 14-3-3 protein in plants, which plays a role in regulating their growth (seed dormancy, cell elongation and division, vegetative and reproduction growth and stress response (salt stress, drought stress, cold stress). Therefore, the 14-3-3 genes are crucial in controlling how plants respond to stress and develop. However, little is known about the function of 14-3-3 gene families in gramineae. In this study, 49 14-3-3 genes were identified from four gramineae, including maize, rice, sorghum and brachypodium, and their phylogeny, structure, collinearity and expression patterns of these genes were systematically analyzed. Genome synchronization analysis showed large-scale replication events of 14-3-3 genes in these gramineae plants. Moreover, gene expression revealed that the 14-3-3 genes respond to biotic and abiotic stresses differently in different tissues. Upon arbuscular mycorrhizal (AM) symbiosis, the expression level of 14-3-3 genes in maize significantly increased, suggesting the important role of 14-3-3 genes in maize-AM symbiosis. Our results provide a better understanding on the occurrence of 14-3-3 genes in Gramineae plants, and several important candidate genes were found for futher study on AMF symbiotic regulation in maize.

Keywords: 14-3-3; AM symbiosis; evolution; gramineous; maize.

Grants and funding

This research was funded by Key research and development Program of Anhui Province (No. 202204c06020021) and National Natural Science Foundation of China (NSFC, No. U21A20235 and No. 31870415).