Data on differential pathogenic ability of Helicobacter pylori isolated from distinct gastric niches

Budhadev Baral; Dharmendra Kashyap; Nidhi Varshney; Tarun Prakash Verma; Ajay Kumar Jain; Debi Chatterji; Vinod Kumar; Amit Mishra; Awanish Kumar; Hem Chandra Jha

doi:10.1016/j.dib.2023.108981

Data on differential pathogenic ability of Helicobacter pylori isolated from distinct gastric niches

Data Brief. 2023 Feb 13:47:108981. doi: 10.1016/j.dib.2023.108981. eCollection 2023 Apr.

Authors

Affiliations

¹ Infection Bioengineering Group, Department of Biosciences and Biomedical Engineering, Indian Institute of Technology Indore, Simrol, Indore, Madhya Pradesh 453552, India.
² Choithram Hospital and research center, Indore, Madhya Pradesh 452014, India.
³ Department of Metallurgy Engineering and Materials Science, Indian Institute of Technology Indore, Simrol, Indore, Madhya Pradesh 453552, India.
⁴ Department of Bioscience & Bioengineering, Indian Institute of Technology Jodhpur, NH 65 Nagaur Road, Karwar, Jodhpur District, Rajasthan 342037, India.
⁵ Department of Biotechnology, National Institute of Technology Raipur, GE Road, Raipur, Chhattisgarh 492010, India.

Abstract

Helicobacter pylori infection is associated with various gastrointestinal diseases and gastric cancer. Our data shows the H. pylori isolates and their associated pathology, isolated from two different stomach niches: gastric epithelium and gastric juice. Gastric adenocarcinoma (AGS) cells were infected with H. pylori juice (HJ1, HJ10 and HJ14) and biopsy (HB1, HB10 and HB14) isolates for 6, 12 and 24 hrs. To determine the cell migration ability of the infected cells, scratch wound assay was performed. The decrease in the wound area was measured by Image J software. Status of cell proliferation accessed by counting the cell number through trypan blue exclusion method. Further assessment of pathogenic potential and carcinogenic ability of the isolates was done by determining the genomic instability in the cell post infection. Cells were stained with DAPI and number of micro and macro nuclei was counted in the acquired images. The data will be helpful in understanding the difference in the carcinogenic ability of H. pylori with respect to their physiological niche.

Keywords: Carcinogenesis; Cell migration; Cell proliferation; Genomic instability; Helicobacter pylori.