High Fischer ratio peptide of hemp seed: Preparation and anti-fatigue evaluation in vivo and in vitro

Zhu Ying; Huang Yuyang; Li Meiying; Sun Bingyu; Liu Linlin; Lv Mingshou; Qu Min; Guan Huanan; Zhu Xiuqing

doi:10.1016/j.foodres.2023.112534

High Fischer ratio peptide of hemp seed: Preparation and anti-fatigue evaluation in vivo and in vitro

Food Res Int. 2023 Mar:165:112534. doi: 10.1016/j.foodres.2023.112534. Epub 2023 Jan 25.

Authors

Zhu Ying¹, Huang Yuyang¹, Li Meiying¹, Sun Bingyu¹, Liu Linlin¹, Lv Mingshou¹, Qu Min¹, Guan Huanan¹, Zhu Xiuqing¹

Affiliation

¹ Department of Food Engineering, Heilongjiang Key Laboratory of Food Science and Engineering, Heilongjiang Key Laboratory of Grain Food and Comprehensive Processing, Harbin University of Commerce, Harbin 150028, Heilongjiang, China.

PMID: 36869539
DOI: 10.1016/j.foodres.2023.112534

Abstract

The high Fischer (F) ratio hemp peptide (HFHP) was prepared by enrichment using activated carbon adsorption, ultrafiltration, and Sephadex G-25 gel filtration chromatography. The OD₂₂₀/OD₂₈₀ ratio reached 47.1 with a molecular weight distribution from 180 to 980 Da, a peptide yield up to 21.7 %, and the F value was 31.5. HFHP had high scavenging ability of DPPH, hydroxyl free radicals, and superoxide. Mice experiments showed that the HFHP increased the activity of superoxide dismutase and glutathione peroxidase. The HFHP had no effect on the body weight of mice, but prolonged their weight-bearing swimming time. The lactic acid, serum urea nitrogen, and malondialdehyde of the mice after swimming was reduced, and the liver glycogen increased. The correlation analysis indicated that the HFHP had significant anti-oxidation and anti-fatigue properties.

Keywords: Anti-fatigue, mice; Anti-oxidation; Fischer ratio; Hemp peptide.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adsorption
Animals
Cannabis*
Chromatography, Gel
Mice
Nitrogen
Peptides
Seeds

Substances

Peptides
Nitrogen