Protective effect of Paecilomyces cicadae TJJ11213 exopolysaccharide on intestinal mucosa and regulation of gut microbiota in immunosuppressed mice

Juanjuan Tian; Xiaogan Zhao; Chao Tang; Xiaomeng Wang; Xueliang Zhang; Luyao Xiao; Wei Li

doi:10.1016/j.foodres.2023.112477

Protective effect of Paecilomyces cicadae TJJ11213 exopolysaccharide on intestinal mucosa and regulation of gut microbiota in immunosuppressed mice

Food Res Int. 2023 Mar:165:112477. doi: 10.1016/j.foodres.2023.112477. Epub 2023 Jan 11.

Authors

Juanjuan Tian¹, Xiaogan Zhao², Chao Tang², Xiaomeng Wang², Xueliang Zhang², Luyao Xiao², Wei Li³

Affiliations

¹ College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China; College of Tea and Food Science Technology, Jiangsu Vocational College of Agriculture and Forestry, Jurong, Jiangsu 212400, PR China.
² College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
³ College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China. Electronic address: lw1981@njau.edu.cn.

PMID: 36869490
DOI: 10.1016/j.foodres.2023.112477

Abstract

The exopolysaccharide (EPS) form Paecilomyces cicadae TJJ1213 possessed immunomodulatory activity in vitro, but whether it could regulate the immune system and intestinal microbiota in vivo remained unknown. In this study, the cyclophosphamide (CTX)-induced immunosuppressive mouse model was established to explore the immunomodulatory activity of EPS. Results showed that EPS could increase the immune organ indices, promote the secretion of serum immunoglobulins and up-regulate the expression of cytokines. Additionally, EPS could repair CTX-induced intestinal injury by increasing the expression of tight junction proteins and promoting the production of short-chain fatty acids. Moreover, EPS could remarkably enhance immunity through TLR4/MyD88/NF-κB and mitogen-activated protein kinase (MAPK) signaling pathways. Furthermore, EPS regulated intestinal microbiota by increasing the abundance of beneficial bacteria (Muribaculaceae, Lachnospiraceae NK4A136, Bacteroides, Odoribacter) and reducing the level of harmful bacteria (Alistipes, Helicobacter). In conclusion, our study suggested that EPS had the abilities to enhance immunity, restore intestinal mucosal injury and modulate intestinal microbiota, and may serve as a potential prebiotic to maintain health in the future.

Keywords: Exopolysaccharide; Gut microbiota; Immunity; Paecilomyces cicadae TJJ1213; Short chain fatty acid.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bacteroides
Bacteroidetes
Gastrointestinal Microbiome*
Intestinal Mucosa
Mice

Supplementary concepts

Cordyceps cicadae