Development of a H2S-responsive NIR Fluorescent Probe for H2S Detection and H2S Releasing Monitoring From Prodrug

Abstract

H₂S was deemed as a toxic gradient in the realm of food and environment but plays pivotal pathophysiological roles in organisms. H₂S instabilities and disturbances are always responsible for multiple disorders. We fabricated a H₂S-responsive NIR fluorescent probe (HT) for H₂S detection and evaluation both in vitro and in vivo. HT exhibited rapid H₂S response within 5 min, accompanied with visible color change and NIR fluorescence generation, and the fluorescent intensities were linearly correlated with corresponding H₂S concentrations. When HT was incubated with A549 cells, the intracellular H₂S and H₂S fluctuations could be monitored ore rotundo via the responsive fluorescence. Meanwhile, when HT was co-administrated with H₂S prodrug ADT-OH, the H₂S release from ADT-OH could be visualized and monitored to evaluate its release efficacy.

Keywords: Fluorescent probe; H2S; H2S prodrug; In vivo imaging; Near-infrared.