Effects of dietary Galla Chinensis tannin supplementation on immune function and liver health in broiler chickens challenged with lipopolysaccharide

Peng Yuan; Haitao Xu; Yuanfei Ma; Jiaxing Niu; Yang Liu; Libo Huang; Shuzhen Jiang; Ning Jiao; Xuejun Yuan; Weiren Yang; Yang Li

doi:10.3389/fvets.2023.1126911

Effects of dietary Galla Chinensis tannin supplementation on immune function and liver health in broiler chickens challenged with lipopolysaccharide

Front Vet Sci. 2023 Feb 14:10:1126911. doi: 10.3389/fvets.2023.1126911. eCollection 2023.

Authors

Peng Yuan¹, Haitao Xu², Yuanfei Ma³, Jiaxing Niu¹, Yang Liu¹, Libo Huang¹, Shuzhen Jiang¹, Ning Jiao¹, Xuejun Yuan⁴, Weiren Yang¹, Yang Li¹

Affiliations

¹ Key Laboratory of Efficient Utilization of Non-grain Feed Resources (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Department of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an, China.
² Animal Husbandry Development Center of Changyi City, Weifang, China.
³ Agricultural and Rural Comprehensive Service Center of Bincheng District, Binzhou, China.
⁴ College of Life Sciences, Shandong Agricultural University, Tai'an, China.

Abstract

Herein, Galla Chinensis tannin (GCT) was examined for its influence on preventing lipopolysaccharide (LPS)-induced liver damage in broiler chickens. Approximately 486 one-day-old healthy broilers were randomly allocated to 3 treatment groups (control, LPS, and LPS + GCT). The control and LPS groups were fed a basal diet and the LPS+GCT group was fed the basal diet supplemented with 300 mg/kg GCT. LPS was intraperitoneally injected (1 mg/kg body weight BW) in broilers in the LPS and LPS+GCT groups at 17, 19, and 21 days of age. The results manifested that dietary GCT addition attenuated LPS-induced deleterious effects on serum parameters and significantly increased serum immunoglobulin and complement C3 concentrations relative to the control and LPS groups. Dietary supplementation of GCT inhibited LPS-induced increase in broiler hepatic inflammatory cytokines, caspases activities, and TLR4/NF-κB pathway-related gene mRNA expression. Therefore, 300 mg/kg GCT addition to the diet improved the immune function of broilers and inhibit liver inflammation by blocking the TLR4/NF-κB pathway. Our findings provide support for the application of GCT in poultry production.

Keywords: Galla Chinensis; broiler; lipopolysaccharide; liver health; tannic acid.

Grants and funding

This research was funded by the Shandong science and technology-based small and medium-sized enterprises innovation capacity improvement project (grant number: 2022TSGC12) and the Starting Research Fund from the Shandong Agricultural University (040/76598).