From start-up to maximum loading: An approach for methane production in upflow anaerobic sludge blanket reactor fed with the liquid fraction of fruit and vegetable waste

Abstract

This investigation provides a reproducible approach for determining the limits of an upflow anaerobic sludge blanket (UASB) reactor designed for the methanization of the liquid fraction of fruit and vegetable waste (FVW_L). Two identical mesophilic UASB reactors were operated for 240 days with a three-day fixed hydraulic retention time and an organic load rate (OLR) increased from 1.8 to 10 gCOD L^-1 d^-1. Because of the previous estimation of flocculent-inoculum methanogenic activity, it was possible to design a safe OLR for the quick start-up of both UASB reactors. The operational variables obtained from the operation of the UASB reactors did not show statistical differences, ensuring the experiment's reproducibility. As a result, the reactors achieved methane yield close to 0.250 L_CH4 gCOD^-1 up to the OLR of 7.7 gCOD L^-1 d^-1. Furthermore, the maximum volumetric methane production rate of 2.0 L_CH4 L^-1 d^-1 was discovered for the OLR ranges between 7.7 and 10 gCOD L^-1 d^-1. The possible overload at OLR of 10 gCOD L^-1 d^-1 resulted in a significant reduction of methane production in both UASB reactors. Based on the methanogenic activity of the UASB reactors sludge, a maximum loading capacity of approximately 8 gCOD L^-1 d^-1 was estimated.

Keywords: Operational strategy. flocculent reactor. high-loading capacity. food waste. overloading. specific methanogenic activity.