The antibacterial activity of Amomum verum Blackw, Zanthoxylum limonella (Dennst.) Alston, Zanthoxylum bungeanum, and Zingiber montanum (J. Koenig) Link ex A. Dietr essential oils were investigated against Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, and Pseudomonas aeruginosa. The essential oils of A. verum Blackw, Z. limonella (Dennst.) Alston, Z. bungeanum, and Z. montanum (J. Koenig) Link ex A. Dietr displayed strong antibacterial activity with a minimum inhibitory concentration and minimumbactericidal concentration ranging from 0.31 to 1.25 µg/mL and 0.62-5.00 µg/mL, respectively. The chemical composition of A. verum Blackw, Z. limonella (Dennst.) Alston, Z. bungeanum, and Z. montanum (J. Koenig) Link ex A. Dietr essential oils were analysed using gas chromatography-mass spectrometry. 1,8-Cineole and limonene were detected in high amounts in the A. verum Blackw and Z. limonella (Dennst.) Alston essential oils, respectively. The major compound in Z. bungeanum and Z. montanum (J. Koenig) Link ex A. Dietr essential oil was 2,4-dimethylether-phloroacetophenone and terpinene-4-ol, respectively. The antibacterial activities and synergistic effects between these essential oils were further analysed. The combination of A. verum Blackw and Z. limonella (Dennst.) Alston essential oils showed a synergistic effect against all bacterial strains, while the other essential oil combinations showed additive, antagonistic effects, and no interaction. The synergistic effect of the combination between A. verum Blackw and Z. limonella (Dennst.) Alston essential oils could be resulted from 1,8-cineole and limonene which was evaluated to possess strong antibacterial activity.
Keywords: Antibacterial activity; Bacterial pathogen; Essential oil; GC–MS; Synergistic effect.
© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.