Protocol for in vivo and ex vivo assessment of hyperglycemia and islet function in diabetic mice

STAR Protoc. 2023 Mar 17;4(1):102133. doi: 10.1016/j.xpro.2023.102133. Epub 2023 Feb 25.

Abstract

Mouse hyperglycemia model and islet function assessment are essential in diabetes research. Here, we provide a protocol to evaluate glucose homeostasis and islet functions in diabetic mice and isolated islets. We describe steps for establishing type 1 and 2 diabetes, glucose tolerance test, insulin tolerance test, glucose stimulated insulin secretion (GSIS) assay, and histological analysis for islet number and insulin expression in vivo. We then detail islet isolation, islet GSIS, β-cell proliferation, apoptosis, and programming assays ex vivo. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022).1.

Keywords: Cell Biology; Cell Isolation; Developmental Biology; Flow Cytometry/Mass Cytometry; Health Sciences; Metabolism; Microscopy; Model Organisms.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Diabetes Mellitus, Experimental*
  • Diabetes Mellitus, Type 1*
  • Diabetes Mellitus, Type 2* / metabolism
  • Disease Models, Animal
  • Glucose / metabolism
  • Hyperglycemia* / diagnosis
  • Hyperglycemia* / metabolism
  • Insulin / metabolism
  • Insulin-Secreting Cells* / metabolism
  • Mice

Substances

  • Glucose
  • Insulin