Nanopore Sequencing for Mixed Samples

Abstract

Long read Nanopore sequencing can be utilised to determine the quality and accuracy of genetically engineered changes in animals, which often produce heterogenous samples. The protocol presented in this chapter can be used for a range of both low and high throughput sequencing applications. DNA must be repaired, barcoded and ligated to sequencing adapters prior to sequencing. Quality of sequencing data produced is dependent on stringent adherence to the protocol. However, nanopore sequencing is a fast moving field, therefore it is worth considering using the most up to date chemistry available.

Keywords: Library preparation; Next-generation sequencing; Oxford Nanopore Technologies; Third-generation nanopore sequencing.