Recombinant Pseudorabies Virus Usage in Vaccine Development against Swine Infectious Disease

Viruses. 2023 Jan 28;15(2):370. doi: 10.3390/v15020370.

Abstract

Pseudorabies virus (PRV) is the pathogen of pseudorabies (PR), which belongs to the alpha herpesvirus subfamily with a double stranded DNA genome encoding approximately 70 proteins. PRV has many non-essential regions for replication, has a strong capacity to accommodate foreign genes, and more areas for genetic modification. PRV is an ideal vaccine vector, and multivalent live virus-vectored vaccines can be developed using the gene-deleted PRV. The immune system continues to be stimulated by the gene-deleted PRVs and maintain a long immunity lasting more than 4 months. Here, we provide a brief overview of the biology of PRV, recombinant PRV construction methodology, the technology platform for efficiently constructing recombinant PRV, and the applications of recombinant PRV in vaccine development. This review summarizes the latest information on PRV usage in vaccine development against swine infectious diseases, and it offers novel perspectives for advancing preventive medicine through vaccinology.

Keywords: pseudorabies virus; recombinant PRV; swine infectious disease; virus modification; virus-vectored vaccines.

Publication types

  • Review
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alphaherpesvirinae*
  • Animals
  • Communicable Diseases*
  • Herpesvirus 1, Suid* / genetics
  • Orthopoxvirus*
  • Pseudorabies* / prevention & control
  • Swine
  • Vaccine Development
  • Vaccines, Combined

Substances

  • Vaccines, Combined

Grants and funding

This work was funded by a grant from the key project of Jiangsu Province’s Key Research and Development Plan (modern Agriculture) (BE2020407), the project of Jiangsu Agri-animal Husbandry Vocational College (NSF2022CB04, NSF2022CB25), the Natural Science Research Project of Higher Education of Jiangsu Province (2020220375), and the Qing Lan Project of Jiangsu Province.