Use of a Longer Aglycon Moiety Bearing Sialyl α(2→3) Lactoside on the Glycopolymer for Lectin Evaluation

Ryota Adachi; Takahiko Matsushita; Tetsuo Koyama; Ken Hatano; Koji Matsuoka

doi:10.3390/polym15040998

Use of a Longer Aglycon Moiety Bearing Sialyl α(2→3) Lactoside on the Glycopolymer for Lectin Evaluation

Polymers (Basel). 2023 Feb 17;15(4):998. doi: 10.3390/polym15040998.

Authors

Ryota Adachi¹, Takahiko Matsushita^{1

2

3}, Tetsuo Koyama¹, Ken Hatano^{1

2

3}, Koji Matsuoka^{1

2

3}

Affiliations

¹ Area for Molecular Function, Division of Material Science, Graduate School of Science and Engineering, Saitama University, Sakura, Saitama 338-8570, Japan.
² Medical Innovation Research Unit (MiU), Advanced Institute of Innovative Technology (AIIT), Saitama University, Sakura, Saitama 338-8570, Japan.
³ Health Sciences and Technology Research Area, Strategic Research Center, Saitama University, Sakura, Saitama 338-8570, Japan.

Abstract

A polymerizable alcohol having 9 PEG repeats was prepared in order to mimic an oligosaccharide moiety. Sialyl α(2→3) lactose, which is known as a sugar moiety of GM3 ganglioside, was also prepared, and the polymerizable alcohol was condensed with the sialyl α(2→3) lactose derivative to afford the desired glycomonomer, which was further polymerized with or without acrylamide to give water-soluble glycopolymers. The glycopolymers had higher affinities than those of glycopolymers having sialyl lactose moieties with shorter aglycon moieties.

Keywords: affinity constant; carbohydrates; fluorescence spectroscopy; glycoclusters; glycopolymers; glycosides; lectins; oligosaccharides; radical polymerizations.

Grants and funding

This research received no external funding.