TM7 (Saccharibacteria) regulates the synthesis of linolelaidic acid and tricosanoic acid, and alters the key metabolites in diapause Clanis bilineata tsingtauica

Lei Qian; Bo-Jian Chen; Pan Deng; Fu-Rong Gui; Ye Cao; Yi Qin; Huai-Jian Liao

doi:10.3389/fphys.2023.1093713

TM7 (Saccharibacteria) regulates the synthesis of linolelaidic acid and tricosanoic acid, and alters the key metabolites in diapause Clanis bilineata tsingtauica

Front Physiol. 2023 Feb 10:14:1093713. doi: 10.3389/fphys.2023.1093713. eCollection 2023.

Authors

Lei Qian¹, Bo-Jian Chen², Pan Deng¹, Fu-Rong Gui³, Ye Cao¹, Yi Qin¹, Huai-Jian Liao^{1

4}

Affiliations

¹ Institute of Leisure Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
² College of Haide, Ocean University of China, Qingdao, China.
³ State Key Laboratory of Conservation and Utilization of Biological Resources of Yunnan, College of Plant Protection, Yunnan Agricultural University, Kunming, China.
⁴ College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, China.

Abstract

Good exploitation and utilization of edible insects can effectively alleviate global food security crisis in years. The study on diapause larvae of Clanis bilineata tsingtauica (DLC) was conducted to explore how gut microbiota regulate the nutrients synthesis and metabolism of edible insects. The results showed that C. bilineata tsingtauica maintained a total and stable nutrition levels at early phase of diapause. The activity of instetinal enzymes in DLC fluctuated markedly with diapause time. Additionally, Proteobacteria and Firmicutes were the predominant taxa, and TM7 (Saccharibacteria) was the marker species of gut microbiota in DLC. Combined the gene function prediction analysis with Pearson correlation analysis, TM7 in DLC was mainly involved in the biosynthesis of diapause-induced differential fatty acids, i.e., linolelaidic acid (LA) and tricosanoic acid (TA), which was probably regulated by changing the activity of protease and trehalase, respectively. Moreover, according to the non-target metabolomics, TM7 might regulate the significant differential metabolites, i.e., D-glutamine, N-acetyl-d-glucosamine and trehalose, via the metabolism of amino acid and carbohydrate pathways. These results suggest that TM7 increased LA and decreased TA via the intestinal enzymes, and altered intestinal metabolites via the metabolism pathways, maybe a key mechanism for regulating the nutrients synthesis and metabolisms in DLC.

Keywords: Clanis bilineata tsingtauica; diapause; edible insects; gut microbes; intestinal metabolites; nutrient synthesis.

Grants and funding

This work was supported by the National Nature Science Foundations of China (NSFC) (32100398), and Jiangsu Agriculture Science and Technology Innovation Fund (JASTIF) (CX(19)2036).