Effects of nitrogen and phosphorus supply levels and ratios on soil microbial diversity-ecosystem multifunctionality relationships in a coastal nontidal wetland

Abstract

Human activities have changed the levels and ratios of nitrogen (N) and phosphorus (P) in wetland ecosystems. However, the effects of N and P levels and ratios on wetland soil microbial community and ecosystem multifunctionality remain unclear, especially on the relationships between soil microbial diversity and ecosystem multifunctionality. In this study, the effects of a 7-year experimental nutrient addition on the soil microbial community and ecosystem multifunctionality (12 function variables related to carbon, N, and P cycling) were assessed by combining three N and P supply levels with three N:P supply ratios in a coastal nontidal wetland ecosystem. According to the obtained results, the N and P supply levels significantly affected soil bacterial community composition, as well as ecosystem multifunctionality, while no significant effects of N:P supply ratios were observed. Although N and P supply levels did not significantly affect bacterial and fungal diversity, they both changed the complexity of bacterial and fungal networks. Soil ecosystem multifunctionality was significantly and positively correlated with bacterial diversity rather than fungal diversity. Moreover, the correlation coefficient between bacterial diversity and ecosystem multifunctionality showed an increasing-decreasing trend with increasing N and P supply levels and an increasing trend with increasing N:P supply ratios. However, the correlation coefficient between bacterial diversity and ecosystem multifunctionality was not significantly correlated with bacterial network complexity. The current study provides new insights into the impacts of N and P levels and ratios on soil microbial community and ecosystem multifunctionality in a coastal nontidal wetland. In particular, the present study highlighted that changes in N and P supply levels and ratios lead to changes in the relationship between soil bacterial diversity and ecosystem multifunctionality, which should be considered in related studies to accurately predict the responses of ecosystem multifunctionality to N and P inputs in coastal nontidal wetlands.

Keywords: Coastal wetland; Fertilization; Microbial diversity; Microbial network; Multifunctionality.