Motility Assays of Chloroflexus

Shin Haruta; Hinata Kakuhama; Shun-Ichi Fukushima; Sho Morohoshi

doi:10.1007/978-1-0716-3060-0_32

Motility Assays of Chloroflexus

Methods Mol Biol. 2023:2646:383-390. doi: 10.1007/978-1-0716-3060-0_32.

Authors

Shin Haruta¹, Hinata Kakuhama², Shun-Ichi Fukushima³, Sho Morohoshi^{2

4}

Affiliations

¹ Department of Biological Sciences, Tokyo Metropolitan University, Hachioji, Tokyo, Japan. sharuta@tmu.ac.jp.
² Department of Biological Sciences, Tokyo Metropolitan University, Hachioji, Tokyo, Japan.
³ SANKEN (Institute of Scientific and Industrial Research), Osaka University, Ibaraki, Osaka, Japan.
⁴ TechnoSuruga Laboratory Co. Ltd., Shizuoka, Japan.

PMID: 36842132
DOI: 10.1007/978-1-0716-3060-0_32

Abstract

Chloroflexus is a thermophilic, filamentous, gliding bacterium. Its multicellular filaments of several hundred micrometer length move straightforward at a speed of approximately 1-3 μm/s and occasionally reverse the moving direction. In liquid media, filaments glide on each other to form cell aggregates without tight adhesion. The molecular machinery on the cell surface that forces the gliding movement has not yet been identified. Here, we describe the cultivation methods to characterize the gliding motility of Chlroflexus and the microscopic assays to determine its gliding speed, reversal frequency, and cell-surface movements.

Keywords: Anoxygenic phototroph; Cell aggregation; Gliding; Multicellular filament; Thermophile.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteria
Cell Membrane
Cell Movement
Chloroflexus*
Movement