Direct Measurement of Kinetic Force Generated by Mycoplasma

Masaki Mizutani; Makoto Miyata

doi:10.1007/978-1-0716-3060-0_28

Direct Measurement of Kinetic Force Generated by Mycoplasma

Methods Mol Biol. 2023:2646:337-346. doi: 10.1007/978-1-0716-3060-0_28.

Authors

Masaki Mizutani¹, Makoto Miyata^{2

3

4

5}

Affiliations

¹ Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan. mizutani-masaki@aist.go.jp.
² Graduate School of Science, Osaka City University, Osaka, Japan.
³ Graduate School of Science, Osaka Metropolitan University, Osaka, Japan.
⁴ The OCU Advanced Research Institute for Natural Science and Technology (OCARINA), Osaka City University, Osaka, Japan.
⁵ The OMU Advanced Research Center for Natural Science and Technology, Osaka Metropolitan University, Osaka, Japan.

PMID: 36842128
DOI: 10.1007/978-1-0716-3060-0_28

Abstract

Optical tweezers enable us to measure the force generated by bacterial motility and motor proteins. Here, we describe a method, using optical tweezers and related techniques, to measure the force generated during Mycoplasma gliding. An avidin-conjugated polystyrene bead trapped by a focused laser beam is bound to the surface-biotinylated Mycoplasma cell, which pulls the bead from the trap center of the laser. The force generated by Mycoplasma is calculated from a displacement measured and a spring constant of the laser trap.

Keywords: Avidin-biotin; Bead; Gliding motility; Optical tweezers; Pathogenic bacteria; Stall force.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Kinetics
Lasers
Mechanical Phenomena
Mycoplasma*
Optical Tweezers