Social Motility Assays of Flavobacterium johnsoniae

Chikara Sato; Keiko Sato

doi:10.1007/978-1-0716-3060-0_23

Social Motility Assays of Flavobacterium johnsoniae

Methods Mol Biol. 2023:2646:287-298. doi: 10.1007/978-1-0716-3060-0_23.

Authors

Chikara Sato^{1

2

3

4}, Keiko Sato⁵

Affiliations

¹ School of Integrative and Global Majors (SIGMA), University of Tsukuba, Tsukuba, Japan.
² Biological Science Course, Graduate School of Science and Engineering, Aoyama Gakuin University, Fuchinobe, Japan.
³ Division of Immune Homeostasis, Department of Pathology and Microbiology, Nihon University School of Medicine, Itabashi, Tokyo, Japan.
⁴ Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Itabashi, Tokyo, Japan.
⁵ Department of Frontier Oral Science, Medical and Dental Sciences, Graduate School of Biomedical Sciences, Nagasaki University, Sakamoto, Nagasaki, Japan. satou@nagasaki-u.ac.jp.

PMID: 36842123
DOI: 10.1007/978-1-0716-3060-0_23

Abstract

Flavobacterium johnsoniae cells move rapidly over solid surfaces by gliding motility. The collective migration of F. johnsoniae on the surfaces results in the formation of spreading colonies. Colony spreading is influenced by adhesin components on the cell surface and the concentrations of agar and glucose. For example, on nutrient-poor agar media, film-like, round spreading colonies are formed. F. johnsoniae displays at least two types of migration: small cell cluster movements leading to concentric colonies and individual cell movements leading to dendritic colonies. The methods for observing colony morphology are described in this chapter.

Keywords: Colony spreading; Gliding motility; Social motility; Surface adhesin.

MeSH terms

Adhesins, Bacterial / metabolism
Agar
Bacterial Proteins* / metabolism
Flavobacterium / metabolism
Locomotion*

Substances

Bacterial Proteins
Agar
Adhesins, Bacterial

Supplementary concepts

Flavobacterium johnsoniae