Twitching Motility Assays of Lysobacter enzymogenes OH11 Under a Light Microscope

Bingxin Wang; Xiaolong Shao; Guoliang Qian

doi:10.1007/978-1-0716-3060-0_19

Twitching Motility Assays of Lysobacter enzymogenes OH11 Under a Light Microscope

Methods Mol Biol. 2023:2646:249-254. doi: 10.1007/978-1-0716-3060-0_19.

Authors

Bingxin Wang¹, Xiaolong Shao¹, Guoliang Qian²

Affiliations

¹ College of Plant Protection, State Key Laboratory of Biological Interactions and Crop Health, Key Laboratory of Integrated Management of Crop Diseases and Pests, Nanjing Agricultural University, Nanjing, People's Republic of China.
² College of Plant Protection, State Key Laboratory of Biological Interactions and Crop Health, Key Laboratory of Integrated Management of Crop Diseases and Pests, Nanjing Agricultural University, Nanjing, People's Republic of China. glqian@njau.edu.cn.

PMID: 36842119
DOI: 10.1007/978-1-0716-3060-0_19

Abstract

Bacterial twitching motility is a peculiar way of adherence and surface translocation on moist solid or semisolid surfaces. Although the twitching motility has been detected in various flagellated bacteria, such as Pseudomonas aeruginosa, it has been rarely detected in flagella-less bacteria like Lysobacter enzymogenes, a natural predator of filamentous fungi. Here, by using a strain OH11 of L. enzymogenes as a model system, we describe a convenient method for observing the twitching motility, with fewer steps and better repetition than conventional methods. This new method provides important technical support for the motile study of Lysobacter.

Keywords: Lysobacter enzymogenes; Surface translocation; Twitching motility; Type IV pili.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins*
Lysobacter*

Substances

Bacterial Proteins

Supplementary concepts

Lysobacter enzymogenes