Site-Directed Cross-Linking Between Bacterial Flagellar Motor Proteins In Vivo

Hiroyuki Terashima; Michio Homma; Seiji Kojima

doi:10.1007/978-1-0716-3060-0_7

Site-Directed Cross-Linking Between Bacterial Flagellar Motor Proteins In Vivo

Methods Mol Biol. 2023:2646:71-82. doi: 10.1007/978-1-0716-3060-0_7.

Authors

Hiroyuki Terashima¹, Michio Homma², Seiji Kojima²

Affiliations

¹ Department of Bacteriology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan. terashima.hiroyuki@nagasaki-u.ac.jp.
² Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya, Japan.

PMID: 36842107
DOI: 10.1007/978-1-0716-3060-0_7

Abstract

The bacterial flagellum employs a rotary motor embedded on the cell surface. The motor consists of the stator and rotor elements and is driven by ion influx (typically H⁺ or Na⁺) through an ion channel of the stator. Ion influx induces conformational changes in the stator, followed by changes in the interactions between the stator and rotor. The driving force to rotate the flagellum is thought to be generated by changing the stator-rotor interactions. In this chapter, we describe two methods for investigating the interactions between the stator and rotor: site-directed in vivo photo-crosslinking and site-directed in vivo cysteine disulfide crosslinking.

Keywords: Bacterial flagellum; Disulfide cross-link; Flagellar motor; FliG; MotA; Photo-cross-link; PomA; Rotary motor; Rotor; Stator.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins* / metabolism
Flagella* / metabolism
Ions / metabolism
Molecular Motor Proteins / metabolism

Substances

Bacterial Proteins
Ions
Molecular Motor Proteins