Unveiling the Anti-Biofilm Property of Hydroxyapatite on Pseudomonas aeruginosa: Synthesis and Strategy

Davoodbasha MubarakAli; Kannappan Arunachalam; Murugan Lakshmanan; Bazigha Badar; Jung-Wan Kim; Sang-Yul Lee

doi:10.3390/pharmaceutics15020463

Unveiling the Anti-Biofilm Property of Hydroxyapatite on Pseudomonas aeruginosa: Synthesis and Strategy

Pharmaceutics. 2023 Jan 30;15(2):463. doi: 10.3390/pharmaceutics15020463.

Authors

Davoodbasha MubarakAli^{1

2

3}, Kannappan Arunachalam⁴, Murugan Lakshmanan¹, Bazigha Badar⁵, Jung-Wan Kim^{2

3}, Sang-Yul Lee²

Affiliations

¹ School of Life Sciences, B.S. Abdur Rahman Crescent Institute of Science and Technology, Chennai 620048, India.
² Centre for Surface Coating and Technology, Department of Material Engineering, Korea Aerospace University, Goyang 10540, Republic of Korea.
³ Division of Bioengineering, Incheon National University, Incheon 22012, Republic of Korea.
⁴ State Key Laboratory of Microbial Metabolism, Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
⁵ Department of Environmental Science, Amar Singh College, Cluster University Srinagar, Srinagar 190008, India.

Abstract

Biofilm-related nosocomial infections may cause a wide range of life-threatening infections. In this regard, Pseudomonas aeruginosa biofilm is becoming a serious health burden due to its capability to develop resistance to natural and synthetic drugs. The utilization of nanoparticles that inhibit biofilm formation is one of the major strategies to control infections caused by biofilm-forming pathogens. Hydroxyapatite (HA) is a synthetic ceramic material having properties similar to natural bones. Herein, a co-precipitation method followed by microwave treatment was used to synthesize HA nanoparticles (HANPs). The resulting HANPs were characterized using X-ray diffraction and transmission electron microscopy. Then, their antibiofilm properties against P. aeruginosa ATCC 10145 were examined in vitro. The needle-shaped HANPs were 30 and 90 nm long in width and length, respectively. The synthesized HANPs inhibited the biofilm formation of P. aeruginosa ATCC 10145 in a concentration-dependent manner, which was validated by light and confocal laser scanning microscopy. Hence, this study demonstrated that HANPs could be used to control the biofilm-related infections of P. aeruginosa.

Keywords: Pseudomonas aeruginosa; anti-biofilm; hydroxyapatite; nanoparticles.

Grants and funding

This study was supported by the INU grant, 2022, and the National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT) (No. 2021R1A2C1010058).