Colonization and Healthcare-Associated Infection of Carbapenem-Resistant Enterobacteriaceae, Data from Polish Hospital with High Incidence of Carbapenem-Resistant Enterobacteriaceae, Does Active Target Screening Matter?

Iwona Pawłowska; Grzegorz Ziółkowski; Estera Jachowicz-Matczak; Michał Stasiowski; Mateusz Gajda; Jadwiga Wójkowska-Mach

doi:10.3390/microorganisms11020437

Colonization and Healthcare-Associated Infection of Carbapenem-Resistant Enterobacteriaceae, Data from Polish Hospital with High Incidence of Carbapenem-Resistant Enterobacteriaceae, Does Active Target Screening Matter?

Microorganisms. 2023 Feb 9;11(2):437. doi: 10.3390/microorganisms11020437.

Authors

Iwona Pawłowska¹, Grzegorz Ziółkowski², Estera Jachowicz-Matczak³, Michał Stasiowski^{4

5}, Mateusz Gajda³, Jadwiga Wójkowska-Mach³

Affiliations

¹ St. Barbara Specialized Regional Hospital No. 5, Division of Microbiology and Epidemiology, 41-200 Sosnowiec, Poland.
² Prof. Kornel Gibiński University Clinical Center, Medical University of Silesia, ul. Medyków 14, 40-572 Katowice, Poland.
³ Department of Microbiology, Faculty of Medicine, Jagiellonian University Medical College, 31-121 Krakow, Poland.
⁴ Chair and Department of Emergency Medicine, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 40-055 Katowice, Poland.
⁵ Department of Anaesthesiology and Intensive Therapy, St. Barbara's Specialized Regional Hospital in Sosnowiec, pl. Medyków 1, 41-200 Sosnowiec, Poland.

Abstract

The objective of the study was to analyse the incidence of carbapenem-resistant Enterobacteriaceae (CRE) at a provincial hospital from 2019-2021. Multiplex PCR was used to detect the presence of carbapenemase genes. There were 399 cases of CRE detected in total in the analysed period, including 104 healthcare-associated infections. Out of the isolated CRE, 97.7% were Klebsiella pneumoniae with OXA-48 or KPC genes. Overall, among the identified CRE genes, the most frequently present genes were the ones mediating oxacillinase OXA-48 (71%) and KPC (26%), and significantly less often New Delhi NDM metallo-β-lactamase (2.5%). Moreover, two isolates produced two carbapenemases, i.e., OXA-48 and KPC. The conducted research demonstrates that there is a constant need for continuous monitoring of the occurrence of CRE strains and the hospital antibiotic policy, as well as the implementation of procedures to prevent CRE transmission by medical personnel and hospital support staff.

Keywords: K. pneumoniae; carbapenemase-producing Enterobacterales; resistance.

Grants and funding

This research was funded by Jagiellonian University Medical College studies number U1C/W41/NO/28.23 and N41/DBS/001019. The APC was funded by Medical University of Silesia, Faculty of Medical Sciences in Zabrze, Katowice, Poland.