Study of the Embryonic Toxicity of TiO2 and ZrO2 Nanoparticles

Elena Nikolaevna Lyashenko; Leyla Djavadovna Uzbekova; Valeri Vladimirovna Polovinkina; Anastasia Konstantinovna Dorofeeva; Said-Umar Sithalil-Ugli Ibragimov; Arslan Ayavovich Tatamov; Albina Gamdullaevna Avkaeva; Anastasia Alekseevna Mikhailova; Inga Shamilevna Tuaeva; Ruslan Kazbekovich Esiev; Sergei Dmitrievich Mezentsev; Marina Alexandrovna Gubanova; Natalya Grigorevna Bondarenko; Alina Yurievna Maslova

doi:10.3390/mi14020363

Study of the Embryonic Toxicity of TiO₂ and ZrO₂ Nanoparticles

Micromachines (Basel). 2023 Jan 31;14(2):363. doi: 10.3390/mi14020363.

Authors

Elena Nikolaevna Lyashenko¹, Leyla Djavadovna Uzbekova², Valeri Vladimirovna Polovinkina¹, Anastasia Konstantinovna Dorofeeva², Said-Umar Sithalil-Ugli Ibragimov¹, Arslan Ayavovich Tatamov³, Albina Gamdullaevna Avkaeva³, Anastasia Alekseevna Mikhailova⁴, Inga Shamilevna Tuaeva⁵, Ruslan Kazbekovich Esiev⁶, Sergei Dmitrievich Mezentsev⁷, Marina Alexandrovna Gubanova⁸, Natalya Grigorevna Bondarenko⁹, Alina Yurievna Maslova^{4

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Affiliations

¹ Department of Obstetrics and Gynecology, Faculty of Pediatrics, S.I. Georgievsky Medical Academy, V.I. Vernadsky Crimean Federal University, 295007 Simferopol, Russia.
² Faculty of Pediatrics, Rostov State Medical University, 344009 Rostov-on-Don, Russia.
³ Faculty of Medicine, Dagestan State Medical University, 367029 Makhachkala, Russia.
⁴ Faculty of Medicine, Stavropol State Medical University, 355017 Stavropol, Russia.
⁵ Department of Hygiene, Faculty of Medicine and Prevention, North Ossetian State Medical Academy, 362019 Vladikavkaz, Russia.
⁶ Department of Dentistry, North Ossetian State Medical Academy, 362019 Vladikavkaz, Russia.
⁷ Department of the National Research, Moscow State University of Civil Engineering, 125009 Moscow, Russia.
⁸ Armavir State Pedagogical University, 352901 Armavir, Russia.
⁹ Department of Philosophy of History of Law, Pyatigorsk Branch of North Caucasus Federal University, 357502 Pyatigorsk, Russia.
¹⁰ SocMedica, 121205 Moscow, Russia.

Abstract

Currently, the widespread use of TiO₂ and ZrO₂ nanoparticles (NPs) in various industries poses a risk in terms of their potential toxicity. A number of experimental studies provide evidence of the toxic effect of TiO₂ and ZrO₂ NPs on biological objects. In order to supplement the level of knowledge and assess the risks of toxicity and danger of TiO₂ and ZrO₂ NPs, we decided to conduct a comprehensive experiment to study the embryonic toxicity of TiO₂ and ZrO₂ NPs in pregnant rats. For the experiment, mongrel white rats during pregnancy received aqueous dispersions of powders of TiO₂ and ZrO₂ NPs at a dose of 100 mg/kg/day. To characterize the effect of TiO₂ and ZrO₂ NPs on females and the postnatal ontogenesis of offspring, a complex of physiological and biochemical research methods was used. The results of the experiment showed that TiO₂ NPs as ZrO₂ NPs (100 mg/kg per os) cause few shifts of similar orientation in the maternal body. Neither TiO₂ NPs nor ZrO₂ NPs have an embryonic and teratogenic effect on the offspring in utero, but both modify its postnatal development.

Keywords: embryotoxicity; nanoparticles; photon correlation spectroscopy; pregnancy; safety; titanium; zirconium.

Grants and funding

This research received no external funding.