Synthesis and Characterization of Calcium Silicate Nanoparticles Stabilized with Amino Acids

Anastasiya A Blinova; Abdurasul A Karamirzoev; Asiyat R Guseynova; David G Maglakelidze; Tatiana A Ilyaeva; Batradz A Gusov; Avetis P Meliksetyants; Mari M Pirumian; Maxim A Taravanov; Maxim A Pirogov; Dmitriy S Vakalov; Tatiana V Bernyukevich; Alexey A Gvozdenko; Andrey A Nagdalian; Andrey V Blinov

doi:10.3390/mi14020245

Synthesis and Characterization of Calcium Silicate Nanoparticles Stabilized with Amino Acids

Micromachines (Basel). 2023 Jan 18;14(2):245. doi: 10.3390/mi14020245.

Authors

Anastasiya A Blinova¹, Abdurasul A Karamirzoev², Asiyat R Guseynova³, David G Maglakelidze¹, Tatiana A Ilyaeva⁴, Batradz A Gusov², Avetis P Meliksetyants², Mari M Pirumian⁵, Maxim A Taravanov¹, Maxim A Pirogov¹, Dmitriy S Vakalov¹, Tatiana V Bernyukevich⁶, Alexey A Gvozdenko¹, Andrey A Nagdalian⁷, Andrey V Blinov¹

Affiliations

¹ Department of Physics and Technology of Nanostructures and Materials, Physical and Technical Faculty, North Caucasus Federal University, 355017 Stavropol, Russia.
² Faculty of Dentistry, North Ossetian State Medical University, 362025 Vladikavkaz, Russia.
³ Faculty of Dentistry, Derzhavin Tambov State University, 392008 Tambov, Russia.
⁴ Faculty of Medicine, Stavropol State Medical University, 355017 Stavropol, Russia.
⁵ Medical and Preventive Faculty, Rostov State Medical University, 344022 Rostov-on-Don, Russia.
⁶ Moscow State University of Civil Engineering, 129337 Moscow, Russia.
⁷ Laboratory of Food and Industrial Biotechnology, North Caucasus Federal University, 355017 Stavropol, Russia.

Abstract

This work presents the development of a method for the synthesis of calcium silicate nanoparticles stabilized with essential amino acids. CaSiO₃ nanoparticles were obtained through chemical precipitation. In the first stage, the optimal calcium-containing precursor was determined. The samples were examined using scanning electron microscopy. It was found that Ca(CH₃COO)₂ was the optimal calcium-containing precursor. Then, the phase composition of calcium silicate was studied using X-ray phase analysis. The results showed the presence of high-intensity bands in the diffractogram, which characterized the phase of the nanosized CaSiO₃-wollastonite. In the next stage, the influence of the type of amino acid on the microstructure of calcium silicate was studied. The amnio acids studied were valine, L-leucine, L-isoleucine, L-methionine, L-threonine, L-lysine, L-phenylalanine, and L-tryptophan. The analysis of the SEM micrographs showed that the addition of amino acids did not significantly affect the morphology of the CaSiO₃ samples. The surface of the CaSiO₃ samples, both without a stabilizer and with amino acids, was represented by irregularly shaped aggregates consisting of nanoparticles with a diameter of 50-400 nm. Further, in order to determine the optimal amino acid to use to stabilize nanoparticles, computerized quantum chemical modeling was carried out. Analysis of the data obtained showed that the most energetically favorable interaction was the CaSiO₃-L-methionine configuration, where the interaction occurs through the amino group of the amino acid; the energy value of which was -2058.497 kcal/mol. To confirm the simulation results, the samples were examined using IR spectroscopy. An analysis of the results showed that the interaction of calcium silicate with L-methionine occurs via the formation of a bond through the NH₃⁺ group of the amino acid.

Keywords: amino acids; calcium silicate; nanoparticles; scanning electron microscopy.

Grants and funding

The study was funded by a grant from the Ministry of Science and Higher Education of the Russian Federation “Study of the mechanisms of interaction of lactic acid microorganisms, lactose-fermenting yeast and biologically active substances during microencapsulation of various fractions of microbiota” by Decree of the Government of the Russian Federation No. 220 in the form of a subsidy from the federal budget for state support of scientific research conducted under the guidance of leading scientists in Russian educational institutions of higher education, scientific institutions and state scientific centers of the Russian Federation (IX turn), Agreement No. 075-15-2022-1129 of 1 July 2022.