Comparative and Functional Analysis of miRNAs and mRNAs Involved in Muscle Fiber Hypertrophy of Juvenile and Adult Goats

Sanbao Zhang; Qiongwen Zhang; Lili Yang; Xiaotong Gao; Ting Chen; Tianbao Li; Wenyue Sun; Yufan Liu; Zihua Zheng; Yan Pan; Yingming Wei; Yanna Huang; Mingsheng Jiang; Qinyang Jiang

doi:10.3390/genes14020315

Comparative and Functional Analysis of miRNAs and mRNAs Involved in Muscle Fiber Hypertrophy of Juvenile and Adult Goats

Genes (Basel). 2023 Jan 25;14(2):315. doi: 10.3390/genes14020315.

Authors

Affiliations

¹ College of Animal Science and Technology, Guangxi University, Nanning 530004, China.
² Institute for New Rural Development, Guangxi University, Nanning 530004, China.
³ College of Animal Science and Technology, Guangxi Agricultural Vocational University, Nanning 530007, China.

Abstract

MicroRNAs (miRNAs) are small non-coding RNAs that post-transcriptionally regulate several pathway intermediates and affect the skeletal muscle development in mice, pigs, sheep, and cattle. However, to date, only a small number of miRNAs have been reported in the muscle development of goats. In this report, the longissimus dorsi transcripts of one- and ten-month-old goats were analyzed by sequencing RNAs and miRNAs. The results showed that the ten-month-old Longlin goats had 327 up- and 419 down-regulated differentially expressed genes (DEGs) compared with the one-month-old. In addition, 20 co-up-regulated and 55 co-down-regulated miRNAs involved in the muscle fiber hypertrophy of goats were identified in ten-month-old Longlin and Nubian goats compared with one-month-old. Five miRNA-mRNA pairs (chi-let-7b-3p-MIRLET7A, chi-miR193b-3p-MMP14, chi-miR-355-5p-DGAT2, novel_128-LOC102178119, novel_140-SOD3) involved in the goat skeletal muscle development were identified by miRNA-mRNA negative correlation network analysis. Our results provided new insight into the functional roles of goat muscle-associated miRNAs, allowing a deeper understanding of the transformation of miRNA roles during mammalian muscle development.

Keywords: goat; hypertrophy; mRNA; miRNA; muscle fiber.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cattle
Gene Expression Profiling
Goats / genetics
Hypertrophy
Mice
MicroRNAs* / genetics
Muscle Fibers, Skeletal / metabolism
RNA, Messenger / genetics
Sheep / genetics
Swine

Substances

MicroRNAs
RNA, Messenger

Grants and funding

This research was funded by the National Key Research and Development Program of China (2021YFD1100100) and Guangxi Innovation Team Construction Project of National Modern Agricultural Industry Technology System (nycytxgxcxtd-2021-09).