Artificial control of mating type and repeated mating to produce polyploid cells in Saccharomyces cerevisiae

Kazumasa Oya; Akira Matsuura

doi:10.1016/j.xpro.2023.102085

Artificial control of mating type and repeated mating to produce polyploid cells in Saccharomyces cerevisiae

STAR Protoc. 2023 Mar 17;4(1):102085. doi: 10.1016/j.xpro.2023.102085. Epub 2023 Feb 2.

Authors

Kazumasa Oya¹, Akira Matsuura²

Affiliations

¹ Department of Biology, Graduate School of Science and Engineering, Chiba University, 1-33 Yayoi-chou, Inage-ku, Chiba 263-8522, Japan.
² Department of Biology, Graduate School of Science, Chiba University, 1-33 Yayoi-chou, Inage-ku, Chiba 263-8522, Japan. Electronic address: amatsuur@faculty.chiba-u.jp.

Abstract

The budding yeast Saccharomyces cerevisiae is an excellent model for examining the effects of ploidy. Here, we provide a protocol for producing polyploid cells by creating a basic unit (matΔ) and polyploidizing it via repeated mating. We describe steps for basic unit construction by one-step transformation, increased ploidy via repeated mating, and ploidy confirmation using flow cytometry. This protocol can be broadly applied to evaluate the physiology of polyploid cells. For complete details on the use and execution of this protocol, please refer to Oya and Matsuura (2022).¹.

Keywords: Cell Biology; Gene Expression; Genetics; Microbiology; Model Organisms; Molecular Biology.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Communication
Flow Cytometry
Humans
Polyploidy*
Reproduction
Saccharomyces cerevisiae* / physiology