Neural progenitor cell-derived extracellular matrix as a new platform for neural differentiation of human induced pluripotent stem cells

Marta S Carvalho; Diogo E S Nogueira; Joaquim M S Cabral; Carlos A V Rodrigues

doi:10.1016/j.bbiosy.2022.100070

Neural progenitor cell-derived extracellular matrix as a new platform for neural differentiation of human induced pluripotent stem cells

Biomater Biosyst. 2022 Nov 11:8:100070. doi: 10.1016/j.bbiosy.2022.100070. eCollection 2022 Dec.

Authors

Marta S Carvalho^{1

2}, Diogo E S Nogueira^{1

2}, Joaquim M S Cabral^{1

2}, Carlos A V Rodrigues^{1

2}

Affiliations

¹ Department of Bioengineering and iBB, Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisbon, Portugal.
² Associate Laboratory i4HB - Institute for Health and Bioeconomy at Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisbon, Portugal.

Abstract

The culture microenvironment has been demonstrated to regulate stem cell fate and to be a crucial aspect for quality-controlled stem cell maintenance and differentiation to a specific lineage. In this context, extracellular matrix (ECM) proteins are particularly important to mediate the interactions between the cells and the culture substrate. Human induced pluripotent stem cells (hiPSCs) are usually cultured as anchorage-dependent cells and require adhesion to an ECM substrate to support their survival and proliferation in vitro. Matrigel, a common substrate for hiPSC culture is a complex and undefined mixture of ECM proteins which are expensive and not well suited to clinical application. Decellularized cell-derived ECM has been shown to be a promising alternative to the common protein coatings used in stem cell culture. However, very few studies have used this approach as a niche for neural differentiation of hiPSCs. Here, we developed a new stem cell culture system based on decellularized cell-derived ECM from neural progenitor cells (NPCs) for expansion and neural differentiation of hiPSCs, as an alternative to Matrigel and poly-l-ornithine/laminin-coated well plates. Interestingly, hiPSCs were able to grow and maintain their pluripotency when cultured on decellularized ECM from NPCs (NPC ECM). Furthermore, NPC ECM enhanced the neural differentiation of hiPSCs compared to poly-l-ornithine/laminin-coated wells, which are used in most neural differentiation protocols, presenting a statistically significant enhancement of neural gene expression markers, such as βIII-Tubulin and MAP2. Taken together, our results demonstrate that NPC ECM provides a functional microenvironment, mimicking the neural niche, which may have interesting future applications for the development of new strategies in neural stem cell research.

Keywords: Decellularized extracellular matrix; Human induced pluripotent stem cells; Neural differentiation; Neural progenitor cells; Stem cell niche.