Candidate gene discovery of Botrytis cinerea resistance in grapevine based on QTL mapping and RNA-seq

Kai Su; Wei Zhao; Hong Lin; Changyue Jiang; Yuhui Zhao; Yinshan Guo

doi:10.3389/fpls.2023.1127206

Candidate gene discovery of Botrytis cinerea resistance in grapevine based on QTL mapping and RNA-seq

Front Plant Sci. 2023 Feb 7:14:1127206. doi: 10.3389/fpls.2023.1127206. eCollection 2023.

Authors

Kai Su^{1

2

3}, Wei Zhao^{1

4}, Hong Lin¹, Changyue Jiang¹, Yuhui Zhao¹, Yinshan Guo^{1

4}

Affiliations

¹ College of Horticulture, Shenyang Agricultural University, Shenyang, China.
² College of Horticulture Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao, China.
³ Hebei Key Laboratory of Horticultural Germplasm Excavation and Innovative Utilization, Qinhuangdao, China.
⁴ National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design and Application Technology (Liaoning), Shenyang, China.

Abstract

Grape gray mold disease (Botrytis cinerea) is widespread during grape production especially in Vitis vinifera and causes enormous losses to the grape industry. In nature, the grapevine cultivar 'Beta ' (Vitis riparia × Vitis labrusca) showed high resistance to grape gray mold. Until now, the candidate genes and their mechanism of gray mold resistance were poorly understood. In this study, we firstly conducted quantitative trait locus (QTL) mapping for grape gray mold resistance based on two hybrid offspring populations that showed wide separation in gray mold resistance. Notably, two stable QTL related to gray mold resistance were detected and located on linkage groups LG2 and LG7. The phenotypic variance ranged from 6.86% to 13.70% on LG2 and 4.40% to 11.40% on LG7. Combined with RNA sequencing (RNA-seq), one structural gene VlEDR2 (Vitvi02g00982) and three transcription factors VlERF039 (Vitvi00g00859), VlNAC047 (Vitvi08g01843), and VlWRKY51 (Vitvi07g01847) that may be involved in VlEDR2 expression and grape gray mold resistance were selected. This discovery of candidate gray mold resistance genes will provide an important theoretical reference for grape gray mold resistance mechanisms, research, and gray mold-resistant grape cultivar breeding in the future.

Keywords: QTL mapping; Vitis vinifera; gray mold; resistance breeding; transcriptome analysis.

Grants and funding

The research was supported by the National Natural Science Foundation of China (Grant No.31972368), the China Agriculture Research System (Grant No. CARS-29-yc-6), the Department of Science and Technology of Liaoning Province (Grant No. 2022030723-JH5/104), the Shenyang Science and Technology Bureau Funds (Grant No. 21-116-3-27) and the Liaoning key R&D Program (Grant No. 2020JH2/10200032).Funding bodies were not involved in the design of the study and collection, analysis, interpretation of data and in writing the manuscript.