lncRNA TPT1-AS1 promotes cell migration and invasion in esophageal squamous-cell carcinomas by regulating the miR-26a/HMGA1 axis

Wenhua Cheng; Fang Yang; Yong Ma

doi:10.1515/med-2022-0533

lncRNA TPT1-AS1 promotes cell migration and invasion in esophageal squamous-cell carcinomas by regulating the miR-26a/HMGA1 axis

Open Med (Wars). 2023 Feb 14;18(1):20220533. doi: 10.1515/med-2022-0533. eCollection 2023.

Authors

Wenhua Cheng¹, Fang Yang², Yong Ma³

Affiliations

¹ The 3rd Department of Digestion, Shanxi Province Cancer Hospital, Shanxi Hospital Affifiliated to Cancer Hospital, Chinese Academy of Medical Sciences, Cancer Hospital Affifiliated to Shanxi Medical University, Taiyuan City, Shanxi Province, 030013, P. R. China.
² Radiotherapy Head and Neck Department, Shanxi Province Cancer Hospital, Shanxi Hospital Affifiliated to Cancer Hospital, Chinese Academy of Medical Sciences, Cancer Hospital Affifiliated to Shanxi Medical University, Taiyuan City, Shanxi Province, 030013, P. R. China.
³ The 2nd Department of Chest Surgery, Shanxi Province Cancer Hospital, Shanxi Hospital Affifiliated to Cancer Hospital, Chinese Academy of Medical Sciences, Cancer Hospital Affifiliated to Shanxi Medical University, No. 3 Workers Xin Jie, Xinghualing District, Taiyuan City, Shanxi Province, 030013, P. R. China.

Abstract

lncRNA TPT1-AS1 plays an oncogenic role in ovarian and cervical cancers. However, its involvement in the pathological progress of esophageal squamous-cell carcinomas (ESCCs) is unclear. lncRNA TPT1-AS1 was mainly localized in the cytoplasm of ESCC cells and interacted with miR-26a. In ESCC tissues, lncRNA TPT1-AS1 level was obviously increased, while miR-26a level was decreased. Interestingly, lncRNA TPT1-AS1 level was not significantly correlated with miR-26a level but was positively correlated with HMGA1 mRNA, a target of miR-26a. In ESCC cell lines KYSE510 and KYSE-30, lncRNA TPT1-AS1 overexpression enhanced HMGA1 expression, while it had no effect on miR-26a expression. Cell migration and proliferation assays indicated that lncRNA TPT1-AS1 and HMGA1 overexpression promoted ESCC cell migration and invasion, while their effects were alleviated by miR-26a overexpression. The migration and invasion of ESCC cells were suppressed by lncRNA TPT1-AS1 knockdown. In conclusion, lncRNA TPT1-AS1 plays an oncogenic role in ESCC and might function by upregulating HMGA1 via sponging miR-26a.

Keywords: HMGA1; esophageal squamous-cell carcinomas; invasion; lncRNA TPT1-AS1; miR-26a; migration.