Application of transposon insertion site sequencing method in the exploration of gene function in microalgae

Xiaobing Hu; Yulong Fan; Chengfeng Mao; Hui Chen; Qiang Wang

doi:10.3389/fmicb.2023.1111794

Application of transposon insertion site sequencing method in the exploration of gene function in microalgae

Front Microbiol. 2023 Feb 3:14:1111794. doi: 10.3389/fmicb.2023.1111794. eCollection 2023.

Authors

Xiaobing Hu^{1

2}, Yulong Fan¹, Chengfeng Mao¹, Hui Chen¹, Qiang Wang^{1

3}

Affiliations

¹ State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University, Kaifeng, China.
² School of Environmental Engineering, Yellow River Conservancy Technical Institute, Kaifeng, China.
³ Academy for Advanced Interdisciplinary Studies, Henan University, Kaifeng, China.

Abstract

Microalgae are a large group of organisms that can produce various useful substances through photosynthesis. Microalgae need to be genetically modified at the molecular level to become "Chassis Cells" for food, medicine, energy, and environmental protection and, consequently, obtain benefits from microalgae resources. Insertional mutagenesis of microalgae using transposons is a practical possibility for understanding the function of microalgae genes. Theoretical and technical support is provided in this manuscript for applying transposons to microalgae gene function by summarizing the sequencing method of transposon insertion sites.

Keywords: chassis cell; flanking sequence; microalgae; sequencing; transposon.

Publication types

Review