Survival on land: A dark-grown seedling searching for path

Hao Gong; Zhiwei Zeng; Léa Tessier; Leno Guzman; Zihao Yuan; Shuai Li; Wenhan Zheng; Ying Chen; Long Qi

doi:10.3389/fpls.2023.1110521

Survival on land: A dark-grown seedling searching for path

Front Plant Sci. 2023 Feb 2:14:1110521. doi: 10.3389/fpls.2023.1110521. eCollection 2023.

Authors

Hao Gong^{1

2}, Zhiwei Zeng³, Léa Tessier⁴, Leno Guzman², Zihao Yuan¹, Shuai Li¹, Wenhan Zheng¹, Ying Chen², Long Qi¹

Affiliations

¹ College of Engineering, South China Agricultural University, Guangzhou, Guangdong, China.
² Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB, Canada.
³ Department of Agricultural Engineering Technology, University of Wisconsin-River Falls, WI, River Falls, United States.
⁴ Department of Biological Sciences, University of Manitoba, Winnipeg, MB, Canada.

Abstract

To initiate its development into a plant, a small dark-grown seedling (prior to its emergence from the ground) must penetrate through the growth media. The path that the seedling takes during this journey has yet to be explained. As such, we conducted non-destructive tests using CT scans to observe the growth of dark-grown seedlings in soil over time; we also developed a model to simulate the dynamics of an emerging seedling, and to examine effects of various growth medium conditions, including Lunar soil. It was previously postulated that, with gravitropism in a terrestrial growth medium, a dark-grown seedling would grow directly upright. However, our CT scan results showed that dark-grown soybean seedlings departed from the vertical path in soil, as far as a lateral distance of approximately 10 mm. The phenomenon of the non-straight path was also demonstrated by the model results. Through simulations, we found that an emerging seedling naturally weaves through the particles of growth medium, in search for the path of least resistance. As a result, the seedling ends up travelling a longer distance. Compared with a seedling that was artificially forced to take a straight path in a growth media, the seedling taking the natural path encountered significantly lower resistances (20% lower) from the growth medium, while travelled 12% longer distance during the emergence process. A seedling encountered a much higher impedance in Lunar soil. Our results suggest that taking the path of least resistance, in addition to shaping and orientating itself for mechanical advantage, are strategies evolved by plant species that have contributed to its vast success. An understanding of plant behavior and survival strategies on Earth lay the foundation for future research in agriculture in novel environments, including on celestial bodies.

Keywords: growth medium; path; pore; resistance; seedling; shape.

Grants and funding

This research was funded by the Laboratory of Lingnan Modern Agriculture Project (NT2021009), the Natural Science Foundation of China (No. 32171904), the Earmarked Fund for Modern Agro-industry Technology Research System (No. CARS-01-47) in China, the Natural Science and Engineering Research Council of Canada (RGPIN-2019-05861), and the China Scholarship Council (No. 202108440352).