Developmental validation of the novel six-dye GoldeneyeTM DNA ID System 35InDel kit for forensic application

Qi Yang; Huan Yu; Yiling Qu; Xiaochun Zhang; Ruocheng Xia; Ziwei Wang; Rui Tan; Lei Xiong; Shihan Xi; Jun Wu; Yuzhen Gao; Suhua Zhang; Chengtao Li

doi:10.1080/20961790.2021.1945723

Developmental validation of the novel six-dye Goldeneye^TM DNA ID System 35InDel kit for forensic application

Forensic Sci Res. 2021 Aug 28;7(4):673-684. doi: 10.1080/20961790.2021.1945723. eCollection 2022.

Authors

Qi Yang^{1

2}, Huan Yu^{1

2}, Yiling Qu^{1

2}, Xiaochun Zhang^{1

2}, Ruocheng Xia^{1

3}, Ziwei Wang^{1

2}, Rui Tan^{1

2}, Lei Xiong^{1

4}, Shihan Xi^{1

5}, Jun Wu⁶, Yuzhen Gao², Suhua Zhang¹, Chengtao Li^{1

2}

Affiliations

¹ Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Sciences, Ministry of Justice, Shanghai, China.
² Department of Forensic Science, Medical School of Soochow University, Suzhou, China.
³ Department of Forensic Medicine, School of Basic Medical Science, Wenzhou Medical University, Wenzhou, China.
⁴ School of Basic Medicine, Inner Mongolia Medical University, Hohhot, China.
⁵ Clinical Medical School, Inner Mongolia University for the Nationalities, Tongliao, China.
⁶ PEOPLESPOTINC, Beijing, China.

Abstract

Insertion/deletion polymorphisms (InDels) have been treated as a prospective and helpful genetic marker in the fields of forensic human identification, anthropology and population genetics for the past few years. In this study, we developed a six-dye multiplex typing system consisting of 34 autosomal InDels and Amelogenin for forensic application. The contained InDels were specifically selected for Chinese population with the MAF ≥ 0.25 in East Asia, which do not overlap with the markers of Investigator^® DIPplex kit. The typing system was named as Goldeneye^TM DNA ID System 35InDel Kit, and a series of developmental validation studies including repeatability/reproducibility, concordance, accuracy, sensitivity, stability, species specificity and population genetics were conducted on this kit. We confirmed that the 35InDel kit is precise, sensitive, species specific and robust for forensic practice. Moreover, the 35InDel kit is capable of typing DNA extracted from forensic routine case-type samples as well as degraded samples and mixture samples. All markers are proved to be highly polymorphic with an average observed heterozygosity (He) of 0.4582. The combined power of discrimination (CPD) is 0.999 999 999 999 978 and the combined power of exclusion in duos (CPE_D) and trios (CPE_T) are 0.978 837 and 0.999573, respectively, which are higher than those of the Investigator^® DIPplex kit. Thus, the Goldeneye^TM DNA ID System 35InDel kit is suitable for forensic human identification and could serve as a supplementary typing system for paternity testing. Supplemental data for this article is available online at https://doi.org/10.1080/20961790.2021.1945723 .

Keywords: 35InDel kit; Forensic sciences; capillary electrophoresis (CE); developmental validation; forensic genetics; insertion and deletion polymorphism (InDel).

Grants and funding

This study was supported by grants from the National Youth Top-Notch Talent of Ten Thousand Program [grant number WRQB2019] and the Youth Science and Technology Innovation Leader of Ten Thousand Program [grant number 2018RA2102], China.