Inhibitory effect of polysaccharides extracted from Changbai Mountain Ganoderma lucidum on periodontal inflammation

Zhen Chen; Wenguang Qin; Hongbing Lin; Yang Liu; Yue Tian; Xuetao Zhao; Tong Ding; Yawei Wang; Tianjiao Mao; Jiang Li; Yuqin Shen

doi:10.1016/j.heliyon.2023.e13205

Inhibitory effect of polysaccharides extracted from Changbai Mountain Ganoderma lucidum on periodontal inflammation

Heliyon. 2023 Jan 28;9(2):e13205. doi: 10.1016/j.heliyon.2023.e13205. eCollection 2023 Feb.

Authors

Zhen Chen¹, Wenguang Qin¹, Hongbing Lin², Yang Liu³, Yue Tian², Xuetao Zhao², Tong Ding², Yawei Wang², Tianjiao Mao¹, Jiang Li¹, Yuqin Shen¹

Affiliations

¹ Department of Periodontics, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, Guangdong, 510182, China.
² Department of Periodontics, School of Stomatology, Jilin University, Changchun, 130021, Jilin Province, China.
³ The Engineering Research Centre of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, Jilin, 130018, China.

Abstract

As the main bioactive substance of Ganoderma lucidum, Ganoderma lucidum polysaccharide (GLP) has anti-inflammatory, antibacterial, and other biological activities. Studies have shown that GLP can regulate the expression of multiple inflammatory cytokines in different inflammatory models and diseases as part of the anti-infection immune response. We extracted crude Changbai Mountain Ganoderma lucidum polysaccharides (CGLPs), analyzed their physical and chemical properties, and then applied them to the periodontitis model to verify whether they have an inhibitory effect on mouse periodontitis. CGLP was determined to be a heteropolysaccharide with dextran as the main component. Its molecular weight was 17.40 kDa. In vivo experiments in mice showed that CGLP can inhibit the alveolar bone loss and reduced inflammation caused of periodontitis by regulating the expression of the inflammatory factors IL-1β, TNF-α, and IL-10 in a concentration-dependent manner.

Keywords: Ganoderma lucidum polysaccharide; Inflammatory cytokine; Periodontitis.