Effects of kiwi fruit (Actinidia chinensis) polysaccharides on metabolites and gut microbiota of acrylamide-induced mice

Mengyin Chen; Xuefeng Chen; Ketang Wang; Luyang Cai; Nannan Liu; Duan Zhou; Wei Jia; Pin Gong; Ning Liu; Yujiao Sun

doi:10.3389/fnut.2023.1080825

Effects of kiwi fruit (Actinidia chinensis) polysaccharides on metabolites and gut microbiota of acrylamide-induced mice

Front Nutr. 2023 Feb 6:10:1080825. doi: 10.3389/fnut.2023.1080825. eCollection 2023.

Authors

Mengyin Chen¹, Xuefeng Chen¹, Ketang Wang¹, Luyang Cai¹, Nannan Liu², Duan Zhou¹, Wei Jia¹, Pin Gong¹, Ning Liu¹, Yujiao Sun¹

Affiliations

¹ School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi'an, China.
² College of Chemistry and Materials Science, Weinan Normal University, Weinan, China.

Abstract

Introduction: Kiwifruit (Actinidia chinensis) has rich nutritious and medicinal properties. It is widely consumed worldwide for the intervention of metabolism disorders, however, the underlying mechanism remains unclear. Acrylamide, a well-known toxic ingredient, mainly forms in high-temperature processed carbohydrate-rich food and causes disorders of gut microbiota and systemic metabolism.

Methods: This study explored the protective effects and underlying mechanisms of kiwifruit polysaccharides against acrylamide-induced disorders of gut microbiota and systemic metabolism by measuring the changes of gut microbiota and serum metabolites in mice.

Results: The results showed that kiwifruit polysaccharides remarkably alleviated acrylamide-induced toxicity in mice by improving their body features, histopathologic morphology of the liver, and decreased activities of liver function enzymes. Furthermore, the treatment restored the healthy gut microbiota of mice by improving the microbial diversity and abundance of beneficial bacteria such as Lactobacillus. Metabolomics analysis revealed the positive effects of kiwifruit polysaccharides mainly occurred through amino and bile acid-related metabolism pathways including nicotinate and nicotinamide metabolism, primary bile acid biosynthesis, and alanine, aspartate and glutamate metabolism. Additionally, correlation analysis indicated that Lactobacillus exhibited a highly significant correlation with critical metabolites of bile acid metabolism.

Discussion: Concisely, kiwifruit polysaccharides may protect against acrylamide-induced toxicity by regulating gut microbiota and metabolism.

Keywords: acrylamide toxicity; gut microbiota; kiwifruit; metabolomics; polysaccharides.

Grants and funding

This research was funded by the National Natural Science Foundation of China (No. 32172171), Science and Technology Innovation Project by Shaanxi Province Department of Agriculture and Rural Affairs (No. NYKJ-2021-XA-003), Key R&D General project of Shaanxi Province Department of Science and Technology (No. 2022NY-014), Xi'an Municipal Science & Technology Bureau (No. 20NYYF0010), National Natural Science Foundation of China (No. 32272428), Young Talent Lifting Project Funded by Shaanxi Association for Science and Technology (No. 20220216), and Special Scientific Research Program of Shaanxi Provincial Education Department (No. 21JK0634).