Migration of tocopherols from the oil phase to the oil-water interface using phospholipids improved the oxidative stability of O/W emulsions

Abstract

The purpose of this research was to effectively migrate tocopherols (T) to the oil-water interface layer (oxidation site) by combining hydrophobic T with amphiphilic phospholipids (P) to improve the oxidative stability of O/W emulsions. Firstly, it was confirmed that the antioxidant ability of TP combinations exhibited synergistic effects in O/W emulsions by measuring lipid hydroperoxides and thiobarbituric acid-reactive species. Moreover, the introduction of P into O/W emulsions to improve the distribution of T at the interfacial layer was confirmed by centrifugation and confocal microscopy methods. Subsequently, the possible mechanisms of synergistic interaction between T and P were described by fluorescence spectroscopy, isothermal titration calorimetry, electron spin resonance, quantum chemical methods and the variation of minor constituents during storage. This research revealed an in-depth insight into the antioxidant interaction mechanism of TP combinations using experimental and theoretical approaches, which provided theoretical guidance for developing emulsion products with better oxidative stability.

Keywords: Interaction; Lipid Oxidation; O/W emulsions; Phospholipid; Tocopherol.