Enzyme-linked immunosorbent assay (ELISA) is by definition a biosensor. However, not all immuno-biosensors involve the use of enzymes, while other biosensors incorporate ELISA as a key signaling component. In this chapter, we review the role of ELISA in signal amplification, integration with microfluidic systems, digital labeling, and electrochemical detection.
Keywords: Amperometric; Analog; Bio-recognition; Biosensor; Digital; Digital ELISA (dELISA); Digital droplet ELISA (ddELISA); Electrochemical; Electrochemiluminescence (ECL); Enzyme-labeled fluorescence (ELF); Impedance; Lab-on-a-chip (LOC); Limit of detection (LOD); Liposome; Luminol; Microfluidics; Minimal detectable dose (MDD); Potentiometric; Pre-equilibrium digital ELISA (PEdELISA); Quantum dots; Ruthenium; Transducer; Tyramide (TSA); Working electrode.
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