Genome-wide identification of the class III peroxidase gene family of sugarcane and its expression profiles under stresses

Heyang Shang; Linqi Fang; Lifang Qin; Hongtao Jiang; Zhenzhen Duan; Hai Zhang; Zongtao Yang; Guangyuan Cheng; Yixue Bao; Jingsheng Xu; Wei Yao; Muqing Zhang

doi:10.3389/fpls.2023.1101665

Genome-wide identification of the class III peroxidase gene family of sugarcane and its expression profiles under stresses

Front Plant Sci. 2023 Jan 30:14:1101665. doi: 10.3389/fpls.2023.1101665. eCollection 2023.

Authors

Heyang Shang^{1

2}, Linqi Fang², Lifang Qin², Hongtao Jiang^{1

2}, Zhenzhen Duan², Hai Zhang¹, Zongtao Yang¹, Guangyuan Cheng¹, Yixue Bao², Jingsheng Xu^{1

2}, Wei Yao², Muqing Zhang^{1

2}

Affiliations

¹ National Engineering Research Center for Sugarcane & Guangxi Key Laboratory of Sugarcane Biology, Fujian Agriculture and Forestry University, Fuzhou, China.
² State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources & Guangxi Key Laboratory of Sugarcane Biology, Guangxi University, Nanning, China.

Abstract

Introduction: Plant-specific Class III peroxidases (PRXs) play a crucial role in lignification, cell elongation, seed germination, and biotic and abiotic stresses.

Methods: The class III peroxidase gene family in sugarcane were identified by bioinformatics methods and realtime fluorescence quantitative PCR.

Results: Eighty-two PRX proteins were characterized with a conserved PRX domain as members of the class III PRX gene family in R570 STP. The ShPRX family genes were divided into six groups by the phylogenetic analysis of sugarcane, Saccharum spontaneum, sorghum, rice, and Arabidopsis thaliana. The analysis of promoter cis-acting elements revealed that most ShPRX family genes contained cis-acting regulatory elements involved in ABA, MeJA, light responsiveness, anaerobic induction, and drought inducibility. An evolutionary analysis indicated that ShPRXs was formed after Poaceae and Bromeliaceae diverged, and tandem duplication events played a critical role in the expansion of ShPRX genes of sugarcane. Purifying selection maintained the function of ShPRX proteins. SsPRX genes were differentially expressed in stems and leaves at different growth stages in S. spontaneum. However, ShPRX genes were differentially expressed in the SCMV-inoculated sugarcane plants. A qRT-PCR analysis showed that SCMV, Cd, and salt could specifically induce the expression of PRX genes of sugarcane.

Discussion: These results help elucidate the structure, evolution, and functions of the class III PRX gene family in sugarcane and provide ideas for the phytoremediation of Cd-contaminated soil and breeding new sugarcane varieties resistant to sugarcane mosaic disease, salt, and Cd stresses.

Keywords: cadmium; class III peroxidase; salt stress; sugarcane; sugarcane mosaic virus.

Grants and funding

This study was supported by the National Natural Science Foundation of China (32001603), the Science and Technology Major Project of Guangxi (Gui Ke AA22117007, Gui Ke AB21238008), and the China Agriculture Research System of MOF and MARA (CARS-170109, CARS-170726). The authors would like to thank the reviewers for their helpful comments on this manuscript.