Detection of carrier Booroola (FecB) allele in BMPR1B gene of MEGA (Merino × Garut) sheep and its association with growth traits

Endang Tri Margawati; Widya Pintaka Bayu Putra; Muhammad Rizki; Edi Soetrisno; Herman Willem Raadsma

doi:10.1186/s43141-023-00475-z

Detection of carrier Booroola (Fec^B) allele in BMPR1B gene of MEGA (Merino × Garut) sheep and its association with growth traits

J Genet Eng Biotechnol. 2023 Feb 15;21(1):19. doi: 10.1186/s43141-023-00475-z.

Authors

Endang Tri Margawati¹, Widya Pintaka Bayu Putra², Muhammad Rizki³, Edi Soetrisno³, Herman Willem Raadsma⁴

Affiliations

¹ Research Center for Applied Zoology, National Research and Innovation Agency (BRIN), Bogor, 16911, Indonesia. endangtri015@gmail.com.
² Research Center for Applied Zoology, National Research and Innovation Agency (BRIN), Bogor, 16911, Indonesia.
³ Department of Animal Science, Faculty of Agriculture, University of Bengkulu, Bengkulu, 38371, Indonesia.
⁴ Center for Advanced Technologies for Animal Genetics and Reproduction, Faculty of Veterinary Science, University of Sydney, Camden, NSW, 2006, Australia.

Abstract

Background: Bone morphogenetic protein receptor 1B (BMPR1B) gene is one of candidate genes for reproductive and growth traits in sheep. The present study was aimed to detect the Booroola (Fec^B) allele in BMPR1B gene and its association with growth traits in MEGA (Merino × Garut) sheep. A total of 82DNA samples collected from individual lamb (mixed-sex) blood were genotyped for allelic polymorphism using a PCR-RFLP method.

Results: The PCR analysis in BMPR1B gene resulted the amplicons with size of140 bp. The RFLP analysis with AvaII restriction enzymeresultedtwo allelic types of wildtype (A/Fec⁺) and mutant or Booroola (G/Fec^B) with frequency of 0.89 and 0.11, respectively. However, the genetic diversity in BMPR1B/AvaII gene of animal studies was categorized tolow category (PIC = 0.18)and under in a genetic equilibrium (χ² = 1.25).

Conclusions: Itshowed us that carrying Fec^B allele in the heterozygous sheep were not associated with growth traits in MEGA sheep.

Keywords: BMPR1B gene; Boorola; Growth traits; MEGA sheep; PCR–RFLP.