Pharmaceuticals have been continuously detected from surface water and groundwater. In order to improve the rejection performance of pharmaceuticals by a nanofiltration membrane (NF), a positively charged membrane was prepared by co-deposition of natural gallic acid and polyethyleneimine on the polyacrylonitrile hydrolysis membrane. Effects of gallic acid concentration, polyethylene imine concentration, reaction time, and the molecular weight of polyethylene imine were documented. The physical and chemical properties of the membrane were also investigated by surface morphology, hydrophilicity, surface charge, and molecular weight cut-off. The optimized membrane had a molecular weight cut-off of about 958 Da and possessed a pure water permeability of 74.21 L·m-2·h-1·MPa-1. The results exhibited salt rejection in the following order: MgCl2 > CaCl2 > MgSO4 > Na2CO3 > NaCl > Na2SO4, while the rejection ability of pharmaceuticals is as follows: amlodipine > atenolol > carbamazepine > ibuprofen, suggesting that the positively charged membrane has enhanced retention to both divalent cations and charged pharmaceuticals. In addition, the antibacterial membrane was obtained by loading silver nanoparticles onto the positively charged membrane, which greatly improved the antibacterial ability of the membrane.