Oxygen saturation in intraosseous sternal blood measured by CO-oximetry and evaluated non-invasively during hypovolaemia and hypoxia - a porcine experimental study

Abstract

Purpose: This study intended to determine, and non-invasively evaluate, sternal intraosseous oxygen saturation (SsO₂) and study its variation during provoked hypoxia or hypovolaemia. Furthermore, the relation between SsO₂ and arterial (SaO₂) or mixed venous oxygen saturation (SvO₂) was investigated.

Methods: Sixteen anaesthetised male pigs underwent exsanguination to a mean arterial pressure of 50 mmHg. After resuscitation and stabilisation, hypoxia was induced with hypoxic gas mixtures (air/N₂). Repeated blood samples from sternal intraosseous cannulation were compared to arterial and pulmonary artery blood samples. Reflection spectrophotometry measurements by a non-invasive sternal probe were performed continuously.

Results: At baseline SaO₂ was 97.0% (IQR 0.2), SsO₂ 73.2% (IQR 19.6) and SvO₂ 52.3% (IQR 12.4). During hypovolaemia, SsO₂ and SvO₂ decreased to 58.9% (IQR 16.9) and 38.1% (IQR 12.5), respectively, p < 0.05 for both, whereas SaO₂ remained unaltered (p = 0.44). During hypoxia all saturations decreased; SaO₂ 71.5% (IQR 5.2), SsO₂ 39.0% (IQR 6.9) and SvO₂ 22.6% (IQR 11.4) (p < 0.01), respectively. For hypovolaemia, the sternal probe red/infrared absorption ratio (SQV) increased significantly from baseline (indicating a reduction in oxygen saturation) + 5.1% (IQR 7.4), p < 0.001 and for hypoxia + 19.9% (IQR 14.8), p = 0.001, respectively.

Conclusion: Sternal blood has an oxygen saturation suggesting a mixture of venous and arterial blood. Changes in SsO₂ relate well with changes in SvO₂ during hypovolaemia or hypoxia. Further studies on the feasibility of using non-invasive measurement of changes in SsO₂ to estimate changes in SvO₂ are warranted.

Keywords: Hypovolaemia; Hypoxia; Oximetry; Pig; Sternum.