Preparation of DRV Liposomes

Sophia G Antimisiaris

doi:10.1007/978-1-0716-2954-3_2

Preparation of DRV Liposomes

Methods Mol Biol. 2023:2622:21-47. doi: 10.1007/978-1-0716-2954-3_2.

Author

Sophia G Antimisiaris^{1

2}

Affiliations

¹ Laboratory of Pharmaceutical Technology, Department of Pharmacy, University of Patras, Patras, Greece. santimis@upatras.gr.
² Institute of Chemical Engineering Sciences, Foundation for Research and Technology Hellas, FORTH/ICE-ΗΤ, Patras, Greece. santimis@upatras.gr.

PMID: 36781747
DOI: 10.1007/978-1-0716-2954-3_2

Abstract

Dried reconstituted vesicle (DRV) liposomes are formulated under mild conditions. The method has the capability to entrap substantially higher amounts of hydrophilic solutes, compared to other passive-loading liposome preparation methods. These characteristics make this liposome type ideal for entrapment of labile substances, such as peptides, proteins, or DNA's (or other nucleotides or oligonucleotides), or in general biopharmaceuticals and sensitive drugs. In this chapter, all possible types of DRV liposomes (in respect to the encapsulated molecule characteristics and/or their applications in therapeutics) are introduced, and preparation methodologies (for each type) are described in detail.

Keywords: Bacteria; Biopharmaceuticals; Cyclodextrin; DNA; DRV; Encapsulation efficiency; Hydrophilic drug; Particulate; Peptide; Protein; Vaccine.

MeSH terms

Hydrophobic and Hydrophilic Interactions
Liposomes* / chemistry
Proteins*

Substances

Liposomes
Proteins