How to Sequence and Assemble Plant Genomes

Abstract

Although nanopore sequencer is a great tool, many plant scientists have suffered from bad sequencing results, even though they have exactly followed the official protocol in preparing a library. This is because the protocol is not optimized for plant genomic DNA. The protocol may be good for sequencing animal or bacterial genomes, but not for plants. However, if the protocol is properly modified, one can obtain lots of long reads and achieve a telomere-to-telomere assembly. Here I present a protocol to that end.

Keywords: Genome assembly; Nanopore sequencing; Organellar genomes; Plant genomes.