qPCR Genotyping of Polyploid Species

Haiyan Wang; Jiangbo Dang; Qigao Guo; Guolu Liang

doi:10.1007/978-1-0716-3024-2_8

qPCR Genotyping of Polyploid Species

Methods Mol Biol. 2023:2638:115-122. doi: 10.1007/978-1-0716-3024-2_8.

Authors

Haiyan Wang¹, Jiangbo Dang¹, Qigao Guo¹, Guolu Liang²

Affiliations

¹ Key Laboratory of Horticulture Science for Southern Mountains Regions of Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Beibei, Chongqing, China.
² Key Laboratory of Horticulture Science for Southern Mountains Regions of Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Beibei, Chongqing, China. lianggl@swu.edu.cn.

PMID: 36781638
DOI: 10.1007/978-1-0716-3024-2_8

Abstract

A simple and cost-effective method for genotyping polyploid plants using quantitative PCR (qPCR) is described in this chapter. There is no additional operation, only simultaneous amplification of alleles and reference sequences with constant copy number in the genome. The qPCR genotyping can detect the genotypes of important traits in polyploid plants without whole genome sequencing data.

Keywords: Allele; Genotyping; Polyploid; Quantitative PCR (qPCR).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alleles
Genome*
Genotype
Genotyping Techniques / methods
Plants / genetics
Polymerase Chain Reaction / methods
Polyploidy*