Improvement of preharvest sprouting resistance with MOTHER OF FT AND TFL 1 + mutated ABA 8'-hydroxylase in white-seeded durum wheat

Yusuke Ban; Keita Kato; Miwako Ito; Mikiko Yanaka; Kanenori Takata

doi:10.1270/jsbbs.22018

Improvement of preharvest sprouting resistance with MOTHER OF FT AND TFL 1 + mutated ABA 8'-hydroxylase in white-seeded durum wheat

Breed Sci. 2022 Dec;72(5):355-361. doi: 10.1270/jsbbs.22018. Epub 2022 Dec 6.

Authors

Yusuke Ban¹, Keita Kato¹, Miwako Ito¹, Mikiko Yanaka¹, Kanenori Takata¹

Affiliation

¹ Western Region Agricultural Research Center, National Agriculture and Food Research Organization (NARO), 6-12-1 Nishifukatsu-cho, Fukuyama, Hiroshima 721-8514, Japan.

Abstract

Improvement of preharvest sprouting (PHS) resistance is an important objective in the breeding of durum wheat (Triticum turgidum ssp. durum (Desf.) Husn.) in Japan, where the harvest timing overlaps with the rainy season. In a previous study, we showed that an R-gene associated with red seed color was the most effective at promoting PHS resistance in durum wheat. However, red-seeded durum wheat is not popular because it discolors pasta. Here, to improve PHS resistance without the R-gene, we introduced a PHS resistance allele of MOTHER OF FT AND TFL 1 (MFT) and a mutated ABA 8'-hydroxylase (ABA8'OH1-A), which is involved in abscisic acid (ABA) catabolism, singly or together into white-seeded durum wheat. The introduction of both genes reliably and stably improved PHS resistance under all tested conditions. Modification of ABA catabolism might be an effective way to improve PHS resistance in durum wheat. Our findings will contribute to improved PHS resistance in breeding for white-seeded durum wheat.

Keywords: ABA; MFT; TaABA8ʹOH1; preharvest sprouting; white-seeded durum wheat.