Sub-lethal effects of spinetoram application interacts with temperature in complex ways to influence respiratory metabolism, life history and macronutrient composition in false codling moth (Thaumatotibia leucotreta)

Elizabeth Huisamen; Henrika J Bosua; Minette Karsten; John S Terblanche

doi:10.1016/j.jinsphys.2023.104490

Sub-lethal effects of spinetoram application interacts with temperature in complex ways to influence respiratory metabolism, life history and macronutrient composition in false codling moth (Thaumatotibia leucotreta)

J Insect Physiol. 2023 Mar:145:104490. doi: 10.1016/j.jinsphys.2023.104490. Epub 2023 Feb 9.

Authors

Elizabeth Huisamen¹, Henrika J Bosua², Minette Karsten³, John S Terblanche⁴

Affiliations

¹ Department of Conservation Ecology and Entomology, Stellenbosch University, South Africa. Electronic address: eopperman@sun.ac.za.
² Department of Conservation Ecology and Entomology, Stellenbosch University, South Africa. Electronic address: henriekb@sun.ac.za.
³ Department of Conservation Ecology and Entomology, Stellenbosch University, South Africa. Electronic address: minettek@sun.ac.za.
⁴ Department of Conservation Ecology and Entomology, Stellenbosch University, South Africa. Electronic address: jst@sun.ac.za.

PMID: 36773842
DOI: 10.1016/j.jinsphys.2023.104490

Abstract

In many pests, insecticide efficacy is dependent on environmental conditions, including ambient temperature. However, it remains unknown if thermal history alters sub-lethal effects to potentially enhance or reduce pesticide resistance in the false codling moth (FCM), Thaumatotibia leucotreta. Here, using FCM, a pest of economic importance in South Africa infesting several commercial food crops, we report results of sub-lethal exposure to spinetoram, an insecticide that disrupts the nervous system. We investigate whether insecticide efficacy is temperature dependent or perhaps interacts with thermal history by testing the effect of a combination of a sub-lethal dose of spinetoram (4 mg/100 ml) and developmental temperature acclimation (22˚C and 28˚C, i.e., a few degrees above or below optimal development temperatures) on the metabolic rate, life history traits and body composition of FCM in the laboratory. A sub-lethal dose of spinetoram reduced metabolic rate of FCM pupae significantly, led to smaller pupal mass and decreased emergence rates. Additionally, males acclimated at 28 °C had a significantly higher emergence rate compared to males acclimated at 22 °C. Body water, body lipids and body protein reserves of adult FCM tended to be higher in the insecticide treatment compared to the control in the 22 °C acclimation group. In the 28 °C acclimation group, body water, lipids and proteins were lower in the insecticide treatment versus the control. Furthermore, sex influenced both emergence rate and body composition with the direction of change depending on insecticide and temperature treatments. Overall, a sub-lethal dose of spinetoram negatively affects body composition and life history traits but interacts with temperature in complex ways. Therefore, both lethal and sub-lethal effects of spinetoram on FCM, in combination with information on the thermal environment experienced by the pest, should be taken into consideration when pest control decisions are made.

Keywords: Developmental acclimation; Energetics; Environmental stress; Macronutrient; Polyphagous; Respiratory metabolism.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Insecticides* / pharmacology
Lipids
Male
Moths* / physiology
Nutrients
Temperature

Substances

spinetoram
Insecticides
Lipids