The protective role of vitamin C on intestinal damage induced by high-dose glycinin in juvenile Rhynchocypris lagowskii Dybowski

Rui Zhu; Zongyu Liu; Minghui Lu; Xueqin Wu; Xueyuan Zhao; Hong-He Wang; Ya-Nan Quan; Li-Fang Wu

doi:10.1016/j.fsi.2023.108589

The protective role of vitamin C on intestinal damage induced by high-dose glycinin in juvenile Rhynchocypris lagowskii Dybowski

Fish Shellfish Immunol. 2023 Mar:134:108589. doi: 10.1016/j.fsi.2023.108589. Epub 2023 Feb 10.

Authors

Rui Zhu¹, Zongyu Liu², Minghui Lu², Xueqin Wu², Xueyuan Zhao², Hong-He Wang², Ya-Nan Quan³, Li-Fang Wu⁴

Affiliations

¹ College of Animal Science and Technology, Jilin Agriculture University, Changchun, 130118, China; Key Laboratory of Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun, 130118, China; Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science, Jilin Agricultural University, Changchun, 130118, China.
² College of Animal Science and Technology, Jilin Agriculture University, Changchun, 130118, China.
³ Jingyuetan Reservoir Management Office, Changchun, 130118, China.
⁴ College of Animal Science and Technology, Jilin Agriculture University, Changchun, 130118, China; Key Laboratory of Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun, 130118, China; Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science, Jilin Agricultural University, Changchun, 130118, China. Electronic address: wulifang8318@163.com.

PMID: 36773713
DOI: 10.1016/j.fsi.2023.108589

Abstract

This study was to evaluate the mitigative effects of vitamin C (VC) on growth inhibition and intestinal damage induced by glycinin in juvenile Rhynchocypris lagowskii Dybowski. 270 healthy juvenile Rhynchocypris lagowskii Dybowski (4.65 ± 0.04 g) were randomly divided into 3 treatments, and fed with control diet, 80 g/kg glycinin diet and 80 g/kg glycinin+200 mg/kg VC diet respectively for 8 weeks. The results showed that glycinin significantly decreased the weight gain rate, specific growth rate, protein efficiency rate, feed efficiency rate and feeding rate of fish compared with the control group (P < 0.05), while VC supplementation improved the growth performance and feed utilization efficiency, and reached a level similar to the control group. Similarly, VC significantly increased the crude protein content of muscle and whole-body, and hepatopancreas and intestinal protease activities of fish fed with glycinin diet (P < 0.05). The distal intestine of fish in glycinin group showed typical damage characteristics, including breakage and atrophy of intestinal mucosal fold, and increased intestinal mucosal permeability. However, fish fed the glycinin + VC diet showed an unimpaired normal intestinal morphology. Usefully, VC supplementation could also restore impaired immune function and antioxidant capacity. VC down-regulated the mRNA levels of pro-inflammatory cytokines TNF-α and IL-1β, and up-regulated the mRNA levels of anti-inflammatory cytokines IL-10 and TGF-β in the distal intestine of fish fed with glycinin. Furthermore, glycinin exposure could reduce the mRNA levels of HO-1, CAT and GPx by inhibiting the activation of Nrf2-Keap1 signaling pathway, while VC supplementation reversed this phenomenon and maintained the homeostasis of antioxidant defense system. Concluded, glycinin causes growth inhibition, digestive dysfunction and intestinal damage of Rhynchocypris lagowskii Dybowski, while sufficient VC intake is beneficial for fish to resist the adverse effects of glycinin.

Keywords: Glycinin; Inflammation; Nrf2-Keap1 signaling pathway; Rhynchocypris lagowskii Dybowski; Vitamin C.

Publication types

Randomized Controlled Trial, Veterinary

MeSH terms

Animal Feed / analysis
Animals
Antioxidants* / metabolism
Ascorbic Acid / pharmacology
Cytokines / metabolism
Diet
Dietary Supplements*
Fish Proteins / genetics
Intestines
Kelch-Like ECH-Associated Protein 1 / metabolism
NF-E2-Related Factor 2 / metabolism
RNA, Messenger / genetics
Vitamins / pharmacology

Substances

Antioxidants
glycinin
Kelch-Like ECH-Associated Protein 1
Ascorbic Acid
NF-E2-Related Factor 2
Vitamins
Cytokines
RNA, Messenger
Fish Proteins