Silk sericin conjugated magnesium oxide nanoparticles for its antioxidant, anti-aging, and anti-biofilm activities

Sushmitha Shankar; Anusha Narayana Murthy; P Rachitha; Vinay B Raghavendra; N Sunayana; Arunachalam Chinnathambi; Sulaiman Ali Alharbi; Nagaraj Basavegowda; Kathirvel Brindhadevi; Arivalagan Pugazhendhi

doi:10.1016/j.envres.2023.115421

Silk sericin conjugated magnesium oxide nanoparticles for its antioxidant, anti-aging, and anti-biofilm activities

Environ Res. 2023 Apr 15:223:115421. doi: 10.1016/j.envres.2023.115421. Epub 2023 Feb 10.

Affiliations

¹ Institute of Excellence, Vijnana Bhavan, University of Mysore, Manasagangotri, Mysore, 570006, India.
² Molecular Biology Department, S-Vyasa, Jigani, Bengaluru, 560105, India. Electronic address: biotechrachitha@gmail.com.
³ P.G. Department of Biotechnology, Teresian College, Siddarthanagar, Mysore, 570011, India. Electronic address: viragh79@gmail.com.
⁴ Microbiology and Cell Biology, Indian Institute of Science, Bangalore, 570012, India.
⁵ Department of Botany and Microbiology, College of Science, King Saud University, PO Box -2455, Riyadh, 11451, Saudi Arabia.
⁶ Department of Biotechnology, Yeungnam University, Gyeongsan, 712-749, Republic of Korea.
⁷ Center for Transdisciplinary Research (CFTR), Department of Pharmacology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India.
⁸ University Centre for Research & Development, Department of Civil Engineering, Chandigarh University, Mohali, India. Electronic address: pugal.smile@gmail.com.

PMID: 36773634
DOI: 10.1016/j.envres.2023.115421

Abstract

The Silk sericin protein was conjugated with magnesium oxide (MgO) nanoparticles to form SS-MgO-NPs . UV, XRD, FTIR, SEM, DLS, and EDX were used to confirm the formation of SS-MgO-NPs. The absorption band of SS-MgO-NPs using UV-visible spectra was observed at 310 nm, with an average size of the nanoparticles was 65-88 nm analyzed from DLS. The presence of alcohol, CN, and CC, alkanes, alkenes, and cis alkenes, in silk sericin, is confirmed by FT-IR and may act as a stabilizing agent. Later SS-MgO-NPs were evaluated for antioxidant, antibacterial, anti-biofilm, ,anti-aging, and anticancer properties. The SS-MgO-NPs inhibited the formation of biofilm of Pseudomonas aeruginosa and Bacillus cereus. The blood compatibility of SS-MgO-NPs, delaying coagulation was observed using human, blood, and goat blood samples. The SS-MgO-NPs exhibited significant anticancer activity on MCF-7 (IC₅₀ 207.6 μg/mL) cancer cell lines. Correspondingly, SS-MgO-NPs demonstrated dose-dependent inhibition of the enzymes in the following order collagenase > elastase > tyrosinase > hyaluronidase, with IC₅₀ values of 75.3, 85.3, 133.6, and 156.3 μgmL^-1, respectively. This exhibits the compoundposses anti-aging properties. So, in in vitro settings, SS-MgO-NPs can be used as an antibacterial, anti-aging, and anticancer agent. Additionally, in vivo research is necessary to validate its therapeutic applications.

Keywords: Silk serecin; anti-aging; anti-biofilm; antioxidant; magnesium oxide.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology
Antioxidants / pharmacology
Biofilms
Humans
Magnesium Oxide / pharmacology
Metal Nanoparticles*
Nanoparticles*
Sericins*
Spectroscopy, Fourier Transform Infrared

Substances

Anti-Bacterial Agents
Antioxidants
Magnesium Oxide
Sericins